摘要
目的:筛选提取柿叶中黄酮的方法及最优提取工艺。方法:以浸膏质量、黄酮含量为指标,比较乙醇回流法、酶法(纤维素酶、β-葡聚糖酶及二者等量混合组成的复合酶)、半仿生法和半仿生酶法(同前3种酶)提取柿叶中总黄酮的效果。以黄酮含量为指标,以料液比、回流温度和回流时间为因素,设计正交试验优化半仿生酶法提取柿叶中黄酮的工艺条件,并进行验证试验。结果:在4种提取方法中,以半仿生酶法得到的浸膏质量和黄酮含量最高,并以复合酶效果最好;优化的半仿生酶法提取工艺条件为料液比1∶14、回流温度50℃、回流时间2.0 h;验证试验中3次黄酮提取率分别为5.9%、5.8%、5.9%(RSD=0.98%,n=3)。结论:优化后的半仿生酶法提取柿叶中黄酮,提取率高且工艺稳定。
OBJECTIVE：To screen the method for extracting total flavonoids in persimmon leaves and optimize extraction tech-nology. METHODS：Using extract quality and flavonoids content as indexes,the effects of extracting total flavonoids in persim-mon leaves by ethanol refluxing method,enzyme method（cellulase,β-glucanase and complex enzyme mixed by equal amounts of both）,semi-bionic method and semi-bionic-enzyme method （the same enzymes） were compared. Using flavonoids content as in-dex,solid-liquid radio,reflux temperature,reflux time as factors,orthogonal test was designed to optimize the extraction technolo-gy conditions of flavonoids in persimmon leaves by semi-bionic-enzyme method,and the verification test was conducted. RE-SULTS：The extract quantity and flavonoids content by semi-bionic-enzyme method was the highest among the 4 extraction meth-ods,and the complex enzyme was the most suitable;the optimized extracting condition of semi-bionic-enzyme method were as fol-lows as solid-liquid radio of 1：14,reflux temperature of 50 ℃,reflux time of 2.0 h;extraction rates of flavonoids in 3 verification tests were 5.9%,5.8%,5.9%（RSD=0.98%,n=3）. CONCLUSIONS：The optimized semi-bionic-enzyme method is efficient and stable in extracting flavonoids in persimmon leaves.
出处
《中国药房》
CAS
北大核心
2017年第13期1813-1816,共4页
China Pharmacy
基金
国家自然科学基金资助项目(No.81172957)
陕西省自然科学基础研究计划项目(No.2016JM8075)
关键词
半仿生酶法
柿叶
黄酮
提取
正交试验
工艺优化
Semi-bionic-enzyme method
Persimmon leaves
Favonoids
Extraction
Orthogonal test
Technology optimization
