摘要
【目的】通过籽瓜花粉的离体培养,比较不同浓度蔗糖、硼酸为基本的液体培养基、不同培养温度以及培养时间对花粉离体萌发的影响。【方法】选用最适培养基,对籽瓜花粉进行6个温度水平以及4个时间水平的离体培养。【结果】当培养温度在25~30℃时的花粉活力最高,籽瓜花粉的最优培养基配方是:15%蔗糖+0.01%硼酸+1%琼脂。【结论】花粉萌发率和花粉管长度均随着温度的上升而增加,在温度上升到30℃以后,随着温度的上升,花粉的萌发率和花粉管长度均降低。在室温下花粉培养4 h时,花粉萌发趋于稳定而花粉管长度仍在增加;培养6 h,其花粉管长度不在增加。
[ Objective] Through the culture of seed -watermelon pollen, the effects of different concentrations of sucrose and boric acid as basic liquid medium at different culture temperatures and in different culture time on pollen germination in vitro are studied. [ Method] In this project, the optimum culture medium was used to carry out in vitro culture at 6 temperature levels and at 4 time levels. [ Result] When the culture temperature was 25 - 30, the highest pollen viability was obtained, and the optimal medium formula was： 15% sucrose, 0.01% boric acid and 1% agar. [ Conclusion] With the increased temperature, the germinal rates of pollen and the length of pollen tube were increased; when the temperature reached up to 30℃, the pollen germination rates and pollen length were both decreased. In addition, the germination rate and pollen length were increased by being cuhured for 4 hours, and then the length was no longer increased after being cultured 6 hours at room temperature.
出处
《新疆农业科学》
CAS
CSCD
北大核心
2017年第3期429-433,共5页
Xinjiang Agricultural Sciences
基金
新疆维吾尔自治区科技支撑项目"不同生态区籽瓜标准化栽培技术研究与示范"(201531101)
新疆维吾尔自治区青年科学基金项目"新疆籽瓜种质资源遗传多样性的ISSR分析"(2015211B014)
新疆农业科学院青年科技基金项目"影响籽瓜花粉活力及结实率温度条件的研究"(xjnky-2014012)~~
关键词
籽瓜
花粉活力
培养基
seed - watermelon
pollen viability
culture medium
