摘要
本研究以常见的抗氧化剂Vc为对照,对橡子壳色素主要成分和体内外抗氧化活性进行了研究分析。结果表明,橡子壳色素中含丰富的多酚类和黄酮类物质,其中总多酚含量达到27.8%,总黄酮含量8.6%。体外抗氧化实验结果表明,橡子壳色素对DPPH·的清除能力和对Fe^(3+)的还原能力均与其质量浓度呈正相关,其中对DPPH·清除率的半数有效剂量(IC_(50)=10μg/m L)大于Vc组(IC_(50)=5μg/m L),当橡子壳色素质量浓度在40μg/m L时达到了Vc的清除能力;当橡子壳色素质量浓度为1 mg/m L时,橡子壳色素还原能力(A710 nm为0.98)接近Vc的还原能力(A710 nm为1.17)。体内抗氧化实验采用对除空白组外的各组实施60Coγ照射进行氧化损伤实验,分析各组的抗氧化能力。结果表明,模型组小鼠体质量和各器官质量与空白组相比显著下降(P<0.05),并且辐照的各组小鼠都出现了食欲不振、精神萎靡等不良现象,表明氧化应激模型建立成功;橡子壳色素组的小鼠器官质量显著高于模型组(P<0.05),低于空白组但无显著差异(P>0.05),该结果表明橡子壳色素对经60Coγ射线照射的小鼠器官有一定的修复作用。实验小鼠血清中的抗氧化性相关酶以及氧化产物的测定结果进一步表明,橡子壳色素低、中、高剂量组均能显著提高小鼠血清中SOD和CAT的活性,并且显著降低MAD含量(P<0.05),其中100 mg/(kg·d)剂量组橡子壳色素的作用效果最好,与同质量浓度Vc作用效果相当。橡子壳色素具有较好的体内和体外抗氧化活性,并且体内100 mg/(kg·d)剂量的橡子壳色素与同剂量下Vc的抗氧化效果相当,作为天然抗氧剂具有较好的应用前景。
Using the common antioxidant Vc as control group,main ingredients and the antioxidant activity in vivo and in vitro of acorn shell pigment were researched in order to provide a theoretical basis for the development and utilization on antioxidant function of acorn shells.The results showed that the acorn shell pigment contained an abundance of polyphenols and flavones,the total content of polyphenols was27.8%,and he total content of flavones was 8.6%.The antioxidant experiment in vitro showed that the DPPH free radical scavenging ability and the Fe3＋reduction ability of acorn shell pigment performed a positive correlation with the increase of its concentration,in which,the half effective dose（IC_（50）=10μg/mL）was higher than Vc group（IC_（50）=5μg/mL）,and it could reach the scavenging ability of Vc once the concentration of acorn shell pigment was around 40μg/mL;when the concentration of acorn shell pigment was 1 mg/mL,the reduction ability of it（A710 nmwas 0.98）was nearly to Vc（A710 nmwas 1.17）.In vivo antioxidant experiment,it used60Coγirradiating the mice to establish an oxidative stress mice model except the blank group to analyze the antioxidant capacity of each group.The results showed that,to compare with the blank group,the body weight and the quality of organs of mice of model group had significantly decreased（P〈0.05）,and it showed a bad phenomenon as loss of appetite,listlessness and so on,which indicated the oxidative stress model was succeed.The organ quality of acorn shell pigment group was significantly higher than the group of mice model（P〈0.05）,but had no significantly different with the below blank group（P〉0.05）.It indicated that the acorn shell pigment had certain repair function to the mice organs irradiated by60COγ.The results of the determination of the antioxidant enzymes and the oxidation products in the serum of the test mice further showed that,the low,medium and high dose groups of acorn shell pigment could all improve the activity of SOD and
出处
《北京林业大学学报》
CAS
CSCD
北大核心
2017年第4期94-100,共7页
Journal of Beijing Forestry University
基金
中央高校基本科研业务费专项(2572014EA02
2572014CA19)
关键词
橡子壳色素
体外抗氧化
体内抗氧化
天然抗氧化剂
acorn shell pigment
antioxidant activity in vitro
antioxidant activity in vivo
natural antioxidant
