摘要
目的通过观察常规交联术后兔眼角膜组织在酶溶液中的变化,评估交联术对角膜耐酶性的影响。方法实验研究。将27只日本大耳白兔随机分成3组(胃蛋白酶组、胰蛋白酶组、胶原蛋白酶组),每组9只,双眼均去上皮,核黄素-右旋糖酐溶液滴眼3 min/次,持续30 min,滴10次。随机取一眼为实验组,另一眼为对照组,实验组兔眼用自研角膜交联机照射30 min,对照组不照射,照射过程中每3 min双眼都用右旋糖酐-核黄素溶液及眼表麻药冲洗1次结膜表面,之后立即处死兔子取双眼角膜,并置于配好的3种对应酶溶液中,每组随机选3只观察并记录每组角膜完全消化的时间及过程。剩下18只兔的眼角膜随机分成3组,每组6只,分别在消化的第1、3、6天随机取出两只兔子剩余的角膜组织作光镜观察(HE染色)。结果每组角膜组织的溶解时间实验组均长于对照组,其中胰蛋白酶组有统计学意义(P<0.05)。从角膜形态变化来看,实验组发生形变的过程均慢于对照组。从光镜观察(HE染色)的结果来看实验组角膜组织发生裂解的速度亦明显慢于对照组。结论角膜交联术能使角膜结构更加稳定、牢固,从而增强其耐酶性。
Objective To assess the effects of corneal cross-linking in rabbit cornea on its resistance to in vitro enzymatic digestion. Methods Twenty-seven Japanese white rabbits were randomly divided into three groups to be treated with different enzymes （pepsin, trypsin, collagenase）. Each group had nine rabbits. Rabbit corneal epithelium was scraped. Riboflavin-dextran solution was topically administrated every three minutes for half an hourl（ i. e, 10 times in total ）. Then one eye （ randomly selected for each animal） was exposed to UV-LED irradiator for 30 minutes. Both eyes were rinsed by dextran-riboflavin and ocular surface anesthetic every 3 minutes during irradiation. Corneas were immediately taken after the rabbits were sacrificed and placed in different enzyme solutions. Time and process until the corneas had been completely digested were recorded. Another 18 Japanese white rabbits served as control. Corneal tissues were put into enzyme solutions without pre-treatment with UV-LED irradiation. Results It took more time to completely digest the cornea tissue from experimental animals, especially by Trypsin （ P 〈 0. 05 ）. Comeal deformation occurs significantly slower. Light microscopy histology showed that the cleavage rate of experimental corneal tissue was significantly slower. Conclusions Corneal cross-linking makes cornea＇ s structure more stable and firmer, thereby enhancing its resistance to enzymatic digestion.
出处
《临床眼科杂志》
2017年第2期165-168,共4页
Journal of Clinical Ophthalmology
基金
武汉市科技攻关计划(No.201161038346)
关键词
角膜交联术
酶
消化
抑制
Comeal collagen cross-linking
Enzyme
Digestion
Resistance
