摘要
目的探讨orexin-B对正常大鼠丘脑底核神经元自发放电及运动行为的影响。方法应用在体电生理细胞外记录的方法观察orexin-B对大鼠丘脑底核神经元自发放电频率的影响,并采用提升躯体摇摆实验(EBST)观察丘脑底核微量注射orexin-B后大鼠运动行为随时间变化。结果大鼠丘脑底核微压力注射orexin-B,神经元放电频率平均升高(96.85±17.42)%(t=10.242,P<0.01),与生理盐水对照组的细胞反应百分数比较差异有极显著性(Z=-4.157,P<0.01)。EBST结果显示,单侧丘脑底核微量注射orexin-B引起大鼠向对侧摇摆,两组在观察的30、45s向对侧偏转的频率比较,差异有显著性(F=80.35,t=13.42、21.54,P<0.01)。结论 Orexin-B可提高正常大鼠丘脑底核神经元兴奋性,提示orexin-B可能通过基底神经节间接通路参与大鼠运动行为调控。
Objective To investigate the effects of orexin-B on spontaneous discharge and locomotive behavior in the subthalamic nucleus of normal rats. Methods Applying in vivo electrophysiological extracellular recording method,the effect of orexin-B on the spontaneous discharge frequency of rat subthalamic nucleus neurons was observed,and adopting elevated body swing tests（EBST）,the changes of motor behavior in rats with time after microinjection of orexin-B into the subthalamic nucleus were observed. Results After micro-pressure injection of orexin-B into rat subthalamic nucleus,neuron discharge average increase of（96.85±17.42）%（t=10.242,P〈0.01）,compared with normal saline group,the difference in percentage of cell response was significant（Z=-4.157,P〈0.01）.EBST results showed that unilateral subthalamic nucleus microinjection of orexin-B caused the rats to swing to the opposite site.The difference in frequency of deflection to the opposite site-in 30 and 45s of observation-between the two groups was significant（F=80.35;t=13.42,21.54;P〈0.01）. Conclusion Orexin-B can increase the excitability of subthalamic nucleus neurons in normal rats,which suggests that orexin-B involves in regulation of motor behavior in rats through indirect pathway of basal ganglia.
作者
盛晴
薛雁
刁汇玲
陈蕾
SHENG Qing XUE Yan DIAO Huiling CHEN Lei(Department of Physiology, Qingdao University Medical Collage, Qingdao 266071, China)
出处
《青岛大学医学院学报》
CAS
2017年第1期25-27,共3页
Acta Academiae Medicinae Qingdao Universitatis
基金
国家自然科学基金资助项目(31671076
310709-42)
青岛市科技局资助项目(14-2-3-1-nsh)
