摘要
目的比较实验用成年和老年猕猴血液生理和生化指标,为今后实验研究提供参考。方法随机选取健康成年猕猴20只、老年猕猴12只,雌雄各半,清醒状态下从后肢隐静脉抽取静脉血,行血常规和血生化检查,从股动脉抽血行血气分析检查。结果在血常规中,老年猕猴的平均红细胞血红蛋白浓度(MCHC)极显著高于成年猕猴(P<0.01);血红蛋白(HCG)、血小板(PLT)、红细胞体积分布宽度标准差(RDW-SD)等显著高于成年猕猴(P<0.05);老年猕猴淋巴细胞计数(LYMPH~#)、嗜酸性粒细胞计数(EO~#)等极显著低于成年猕猴(P<0.01);白细胞计数(WBC)显著低于成年猕猴(P<0.05)。血生化中,老年猕猴的单胺氧化酶(MAO)、甘油三脂(TRIG)极显著高于成年猕猴(P<0.01);丙氨酸转氨酶(ALT)、血糖(GLU)、乳酸脱氢酶(LDH)显著高于成年猕猴(P<0.05);老年猕猴的超氧化物歧化酶(SOD)极显著低于成年猕猴(P<0.01)。动脉血气中,老年猕猴的血乳酸(Lac)极显著高于成年猕猴(P<0.01);老年猕猴的二氧化碳分压(PaCO_2)、碳酸氢根(HCO_3^+)、二氧化碳总量(TCO_2)、碱剩余(BE)等极显著低于成年猕猴(P<0.01)。结论成年和老年猕猴某些血液学指标有显著性差异,进行生物医学研究时应予以充分考虑。
Objective To analyze and compare the hematological and serum biochemical status between adult rhesus monkeys （Macaca mulatta） and aged rhesus monkeys （Macaca mulatta）. Method Blood samples were obtained from 20 healthy adult rhesus monkeys and 12 aged healthy rhesus monkeys（ half male and half female）. All the samples were tested in the automatic blood cellular analyzer, automatic blood biochemical analyzer and automatic blood gas analyzer. Result Compared with the adult rhesus monkeys, hematologic values of the aged monkeys such as MCHC （ P 〈 0.01 ） , HCG, PLT, RDW-SD （ P 〈 0.05 ） , were significantly higher, while other hematologic values such as LYMPH＂, EO＂ （P 〈 0.01 ） , WBC （P 〈 0.05 ） , were significantly lower. In serum biochemistry test, values of the aged rhesus monkeys such as MAO, TRIG （ P 〈 0.01 ） , ALT, GLU, LDH （ P 〈 0.05） , were significantly higher than the correspond value of the adult rhesus monkeys, while other values such as SOD（P 〈0.01） were significantly lower. In artery blood gas analysis, compared with the adult rhesus monkeys, the aged monkeys＇Lac values were significantly higher（P 〈0.01） , while values such as PaCO2, HCO3＋ , TCO2, BE were lower （P 〈 0.01 ）. Conclusion There are some significantly differences in hematological and serum biochemical values between adult and aged rhesus monkeys, and we should take this into full consideration during biochemical or medical research.
作者
张永一
赵维星
王显望
张晓莹
曹江北
米卫东
ZHANG Yong-yi ZHAO Wei-xing WANG Xian-wang ZHANG Xiao-ying CAO Jiang-bei MI Wei-dong(General Hospital of Peoples Liberation Army, Anesthesia and Operation Center, Beijing 100853, China NO. 309 Hospital of People＇s Liberation Army, Beijing 100091, China)
出处
《实验动物科学》
2017年第1期37-40,共4页
Laboratory Animal Science
基金
总后十二五创新专项基金(No.13CXZ034)
总后十二五重点课题(No.BWS11C024)
关键词
成年猕猴
老年猕猴
血常规
血液生化
血气分析
adult rhesus monkey
aged rhesus monkey
hematology
serum biochemistry
blood gas analysis
