摘要
目的:探讨苗药头花蓼(polygonum capitatum)对幽门螺杆菌(Helicobacter pylori,H.pylori)感染慢性胃炎大鼠胃黏膜及血清白介素(interleukin,IL)-17和IL-23表达水平的影响。方法:SD大鼠分为空白组、模型组和头花蓼组,后两组大鼠采用H.pylori标准菌灌胃SD大鼠建立H.pylori慢性胃炎大鼠模型,头花蓼组大鼠在造模成功后给予头花蓼1.72 g浸膏/(kg·d)的剂量灌胃给药2周,4周后取3组大鼠腹主动脉血及胃黏膜,HE染色观察大鼠胃黏膜病理学改变,酶联免疫法(ELISA)检测血清IL-17与IL-23含量,实时免疫荧光定量PCR(Real time-PCR,RT-PCR)检测胃黏膜IL-17 mRNA与IL-23 mRNA相对表达水平。结果:与模型组比较,头花蓼组胃黏膜炎症明显减轻,病理评分显著降低(P<0.05);与模型组比较,头花蓼组大鼠血清IL-17及胃黏膜IL-17 mRNA相对表达水平明显降低(P<0.05),血清IL-23含量明显降低(P<0.05)。结论:头花蓼可减轻H.pylori感染所致胃黏膜炎性损伤,此保护作用可能与抑制IL-17及IL-23的分泌有关。
Objective： To investigate the effects ofpolygonum capitatum on IL-17 and IL-23 levels in rats with Helicobacter pylori （H. pylori）-induced gastritis. Methods： Rats were derided into three groups including blank group, model group and polygounum capitatum treating group. The rats in the latter two groups were treated with H. pylori standard bacteria to establish the model of H. pylori chronic gastritis. After the modeling, the medication group was given polygonum capitatum with the dose of 1.72 g extract/（ kg · d） by gavage for 2 weeks. Then normally feeding 2 weeks later, after that, rats were sacrificed, the gastric mucosa and abdominal aortic blood were collected, pathological changes were examined by HE staining,the levels of serum IL-17 and IL-23 were measured by enzyme-linked immunosorbent assay. The relative transcript levels of IL-17 and IL-23 mRNA in gastric mucosa were determined by real time PCR. Rusult： Compared with model group, the gastritis in treatment group alleviated remarkably and the pathological scores decreased statistically. The level of serum IL-17 and relative expression of mucosa IL-17 mRNA reduced obviously after polygonum capitatum therapy as well. The level of serum IL-23 also significantly decreased in polygonum capitatum group. Conclusion： Polygonum capitatum could reduce H. pylori-induced gastritic mucosal injury, which may be related to inhibiting IL-17 and IL-23 secretion.
出处
《贵州医科大学学报》
CAS
2017年第4期421-425,430,共6页
Journal of Guizhou Medical University
基金
贵州省科学技术基金[黔科合(2014)2027号]
贵州省科技合作计划基金[黔科合LH(2015)7417号]
贵州省卫生计生委科学技术基金(gzwikj2015-1-003)
贵阳市科技局计划项目[筑科合同(2014001)]
