摘要
目的:观察芒果苷联合葛根素对自发性高血压大鼠(spontaneous hypertensive rats,SHR)心肌炎性损伤的协同保护作用,为高血压病心肌炎性损伤的临床防治提供实验依据。方法:以8只正常血压大鼠(normal blood pressure rat,WKY)为空白组A,72只10周龄SHR大鼠随机分为模型组B,苯那普利(10 mg·kg^(-1)·d^(-1))组C,芒果苷单用(20 mg·kg^(-1)·d^(-1))组D,葛根素单用组E(20 mg·kg^(-1)·d^(-1)),芒果苷(20 mg·kg^(-1)·d^(-1))+葛根素片(10 mg·kg^(-1)·d^(-1))组F,芒果苷(20 mg·kg^(-1)·d^(-1))+葛根素片(20 mg·kg^(-1)·d^(-1))组G,芒果苷(20 mg·kg^(-1)·d^(-1))+葛根素片(40 mg·kg^(-1)·d^(-1))组H,芒果苷(10 mg·kg^(-1)·d^(-1))+葛根素片(20 mg·kg^(-1)·d^(-1))组I,芒果苷(40 mg·kg^(-1)·d^(-1))+葛根素片(20 mg·kg^(-1)·d^(-1))组J。每天10 m L·kg-1连续灌胃,灌胃给药2个月后停药,取材前禁食12 h。用显微镜观察大鼠心肌组织病理形态结构,免疫组化法检测大鼠心肌组织白细胞介素-6(interleukin-6,IL-6),白细胞介素-10(interleukin-10,IL-10),肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)的表达,酶联免疫法检测SHR心肌组织IL-6,IL-10,TNF-α水平。结果:各组大鼠心脏组织均未见明显病理形态学改变;免疫组化和酶联免疫结果显示,与模型组比较,各组给药大鼠心肌组织IL-6,IL-10,TNF-α含量均有不同程度下降(P<0.05,P<0.01)。结论:芒果苷联合葛根素对自发性高血压大鼠心脏组织病理形态学无显著影响;但可下调大鼠心脏组织异常升高的炎症因子IL-6,IL-10,TNF-α水平,具有一定的协同增效作用。
Objective： To observe the synergistic protective effect of mangiferin combined with puerarin on spontaneously hypertensive rat（ SHR） inflammation injury,in order to provide the experimental basis for clinical prevention and treatment of hypertensive patients with myocarditis induced by hypertension. Method： Eight rats with normal blood pressure rat（ WKY） were taken as blank group（ A）; 72 10-week-old SHR rats were taken as the study object and randomly divided into model group（ B）,benazepril group（ C,10 mg·kg^-1·d^-1,single mangiferin group（ D,20 mg·kg^-1·d^-1,single puerarin group（ E,20 mg·kg^-1·d^-1,mangiferin（ 20 mg·kg^-1·d^-1 ＋ puerarin（ 10 mg·kg^-1·d^-1 group（ F）,mangiferin（ 20 mg·kg^-1·d^-1 ＋ puerarin（ 20 mg·kg^-1·d^-1group（ G）,mangiferin（ 20 mg·kg^-1·d^-1 ＋ puerarin（ 40 mg·kg^-1·d^-1 group（ H）,mangiferin（ 10 mg·kg^-1·d^-1 ＋ puerarin（ 20 mg·kg^-1·d^-1 group（ I）,and mangiferin（ 40 mg·kg^-1·d^-1 ＋ puerarin（ 20 mg·kg^-1·d^-1 group（ J）. The rats were orally administered with drugs（ 10 m L·kg- 1） for consecutively two months. They were fasting for 12 h before being put to death. The morphology of myocardial tissues in SHR was observed under microscope. Immunohistochemical assay and enzymelinked immunosorbent assay（ ELISA） were used to detect interleukin（ IL）-6,IL^-10 and tumor necrosis factor-α（ TNF-α）. Result： Heart tissues of rats of each group showed no significant morphological change; immunohistochemistry and ELISA results showed that compared with the model group,each drug treated group showed decreases in myocardial IL-6,IL^-10,TNF-α levels to varying degrees（ P〈0. 05,P〈0. 01）. Conclusion： Mangiferin combined with puerarin have no significant effect on morphology of heart tissues of SHR,but can down-regulate abnormal elevation of inflammatory cytokines IL-6,IL^-10,TNF-α in heart tissues.
出处
《中国实验方剂学杂志》
CAS
CSCD
北大核心
2017年第9期146-151,共6页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广西科技基础条件平台项目(10-046-04)
关键词
自发性高血压大鼠
芒果苷
葛根素
炎性损伤
协同作用
spontaneous hypertensive rat
mangiferin
puerarin
inflammatory injury
synergistic protective effect
