摘要
目的:探讨超声造影对胃癌患者病灶中癌细胞增殖、侵袭功能及血管新生程度的评估价值。方法:纳入2012年8月~2016年5月本院收治的胃癌患者39例作为胃癌组,胃溃疡患者48例作为胃溃疡组,另取同期在本院接受胃镜检查的健康人50例作为正常对照组。入院后当日,应用彩色多普勒超声仪检测胃部超声造影参数;采用荧光定量PCR法检测胃部组织增殖、侵袭基因mRNA表达量;采用酶联免疫吸附法(ELISA)检测血清血管新生指标含量。结果:胃癌组、胃溃疡组患者的超声造影参数增强时间(ET)、峰值时间(TTP)显著低于正常对照组,胃癌组患者的超声造影参数ET、TTP显著低于胃溃疡组;胃癌组患者组织中Stat3、Survivin、Bcl-2、β-catenin、eIF4E、CD44、UHRF1、c-met的mRNA表达量以及血清中血管内皮生长因子(VEGF)、表皮生长因子受体(EGFR)、低氧诱导因子-1α(HIF-α)、血管生成素-2(Ang-2)的含量均高于胃溃疡组和正常对照组,组织中E-cadherin的mRNA表达量低于胃溃疡组和正常对照组;Spearman相关性分析显示,超声造影参数ET、TTP水平与病灶中癌细胞增殖、侵袭功能及血管新生指标呈相关关系。结论:超声造影参数可准确评估胃癌恶性程度,有望成为日后胃癌早期诊断及治疗指导的可靠手段。
Objective：To study the value of ultrasound contrast for assessing cancer cell proliferation and invasion as well as angiogenesis in lesions of in patients with gastric cancer.Methods：A total of 39 patients with gastric cancer and 48 patients with gastric ulcer who were treated in our hospital between August 2012 and May 2016 were included in gastric cancer group and gastric ulcer group respectively,and 50 healthy subjects who accepted gastroscopy in our hospital during the same period were included in normal control group.The day after admission,color Doppler diasonograph was used to test the gastric ultrasound contrast parameters;fluorescence quantitative PCR method was used to detect the proliferation and invasion gene mRNA expression in stomach tissue;enzyme-linked immunosorbent assay（ELISA）was used to detect the serum angiogenesis index levels.Results：Ultrasound contrast parameters ET and TTP levels of gastric cancer group and gastric ulcer group were significantly lower than those of normal control group,and ultrasound contrast parameters ET and TTP levels of gastric cancer group were significantly lower than those of gastric ulcer group;Stat3,Survivin,Bcl-2,β-catenin,eIF4E,CD44,UHRF1 and c-met mRNA expression in tissue as well as VEGF,EGFR,HIF-αand Ang-2 levels in serum of gastric cancer group were higher than those of gastric ulcer group and normal control group while E-cadherin mRNA expression in tissue was lower than those of gastric ulcer group and normal control group;Spearman correlation analysis showed that ultrasound contrast parameters ET and TTP levels were correlated with the cancer cell proliferation and invasion function as well as angiogenesis indexes in lesions.Conclusions：Ultrasound contrast parameters can accurately assess the malignant degree of gastric cancer,and is expected to become the reliable means for early diagnosis and treatment guidance of gastric cancer in the future.
出处
《海南医学院学报》
CAS
2017年第6期858-861,864,共5页
Journal of Hainan Medical University
基金
四川省科技攻关计划项目(2005sz01357)~~
关键词
胃癌
超声造影
增殖
侵袭
血管新生
Gastric cancer
Ultrasound contrast
Proliferation
Invasion
Angiogenesis
