摘要
目的:探讨血小板衍生生长因子(PDGF-BB)对体外粒-单核细胞集落形成单位(CFU-GM)、巨核细胞集落形成单位(CFU-MK)的生成作用及其对人巨核细胞株CHRF抗凋亡作用的具体机制。方法:采用血浆凝固培养法培养小鼠和人骨髓细胞CFU-GM和CFU-MK,探讨PDGF-BB对两者CFU-GM、CFU-MK形成能力的影响。通过AnnexinV/PI双染、活性Caspase-3表达及JC-1线粒体膜势能流式细胞术检测研究PDGF-BB对人巨核细胞株CHRF的抗凋亡作用机制。结果:PDGF-BB 0-100 ng/ml能够促进小鼠/人CFU-GM和CFU-MK的增殖并呈剂量依赖性,其中50 ng/ml作用最为明显(P<0.01)。流式细胞术检测结果表明,PDGF-BB通过线粒体及Caspase-3通路对人巨核细胞株CHRF发挥抗凋亡作用(P<0.05)。结论:PDGF-BB能够促进体外小鼠和人粒-单核系及巨核系造血生成,且能通过线粒体途径和Caspase-3通路对巨核细胞发挥抗凋亡作用。
Objective： To investigate the effect of platelet-derived growth factor （PDGF-BB） on the formation of granulocyte-monocyte colony forming unit （CFU-GM） and megakaryocyte colony forming unit （CFU-MK） and its anti-apoptotic effect on CHRF cells. Methods： The CFU-GM and CFU-MK of murine and human bone marrow cells were cultured in vitro by using plasma clot culture system. The anti-apoptotic effect of PDGF-BB on CHRF cells and its mechanism were clarified by flow cytometry. Results： PDGF-BB 0 - 100 ng/ml stimulated the proliferation of murine and human CFU-GM and CFU-MK in a dose-dependent manner. The maximal stimulation effect was obtained at 50 ng/ ml of PDGF-BB （ P 〈 0. 01 ）. Furthermore, PDGF-BB had an anti-apoptotic effect on CHRF cells as shown by the flow cytometry with AnnexinV/PI double staining, Caspase-3 expression and JC-1 detection （ P 〈 0. 05 ）. Conclusion： PDGF-BB significantly stimulates the proliferation of CFU-GM and CFU-MK in vitro, and has an anti - apoptotic effect on CHRF cells.
出处
《中国实验血液学杂志》
CAS
CSCD
北大核心
2017年第2期555-561,共7页
Journal of Experimental Hematology
基金
国家自然科学基金(81270580)
广东省自然科学基金(2014A030313315)
