摘要
目的研究单宁酸(tannic acid,TA)对电离辐射所致巨核细胞损伤的保护作用,并探讨其作用机制。方法体外培养巨核细胞株M07e,经0、0.5、1、2.5、5、10μg/m L单宁酸预处理后给予10 Gy60Coγ射线照射,继续培养不同时间,采用MTT法、荧光酶标仪检测法和流式细胞术检测技术分别检测细胞存活率、活性氧(ROS)水平、线粒体膜电位(mitochondrial membrane potential,MMP)和凋亡率变化。给予BALB/c小鼠5 Gy60Coγ射线全身一次性照射,复制急性放射损伤实验动物模型,设置生理盐水对照组和TA灌胃预处理组,通过尾静脉外周血常规检测和骨髓病理切片观察,分析血小板水平和骨髓巨核细胞数目变化情况。结果 TA能够剂量依赖性地提高受照巨核细胞的存活率;与单纯辐照组相比,TA预处理可以显著降低细胞内总ROS水平(P<0.01)、提高细胞线粒体膜电位(P<0.05)和降低细胞凋亡率(P<0.05)。动物实验结果显示,TA预处理能够显著抑制辐射损伤引起的骨髓巨核细胞数量减少,照后第7、12、15、19天时TA处理组血小板水平显著高于对照组(P<0.05,P<0.01)。结论 TA对电离辐射所致巨核细胞损伤具有显著保护作用,其作用机制可能与其突出的抗氧化应激活性有关。
Objective To determine the protective effect of tannic acid （TA） on megakaryocytes against ionizing radiation and investigate its underlying mechanism. Methods Human megakaryoblast M07e cells were pretreated with TA at concentrations of 0, 0. 5, 1,2.5, 5 and 10 μg/mL before receiving 10 Gy γ- ray irradiation. The cell viability, levels of reactive oxygen species （ROS） , mitochondrial membrane potential （MMP） and apoptotic rate of the cells were detected after irradiation. Subsequently, BALB/c mice with or without TA treatment were irradiated with 6o Co ~/at a dose of 5 Gy, and the blood platelet count and number of megakaryocytes in bone marrow were determined at appointed time points. Results TA dose-dependently improved the survival rate of M07e cells after irradiation. Compared with control group, TA significantly reduced the intracellular ROS levels （ P 〈 0.05 ）, improved MMP （ P 〈 0.05 ） and decreased the apoptotic rate （P 〈0.05） in M07e cells after irradiation. In vivo, TA pretreatment resulted in a significant increase in the number of megakaryocytes in bone marrow in BALB/c mice after irradiation. Meanwhile, the blood platelet counts in TA treated mice were much higher than those in the control group, especially at days 7,12,15, and 19 after irradiation （P 〈 0. 05, P 〈 0. 01 ）. Conclusion TA has a protective effect on megakaryocytes against ionizing irradiation, possibly via its inhibition on radiation-induced oxidative stress.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2015年第7期638-642,共5页
Journal of Third Military Medical University
基金
全军后勤科研"十二五"计划课题重点项目(BWS11J009)
新药创制科技重大专项(2013ZX09J13101-03B)~~
关键词
单宁酸
巨核细胞
血小板
辐射损伤
tannic acid
megakaryocyte
platelet
radiation injury
