摘要
目的建立骨折合剂中没食子酸、紫丁香苷、绿原酸、咖啡酸、芍药苷和丹皮酚6种化学成分的HPLC测定方法。方法采用Hypersil ODS色谱柱(250 mm×4.6 mm,5μm)进行分离,流动相为0.4%甲酸水溶液-乙腈,梯度洗脱,检测波长为254 nm,流速为1.0 m L·min^(-1),柱温为30℃,进样量5μL。结果没食子酸、紫丁香苷、绿原酸、咖啡酸、芍药苷和丹皮酚线性范围分别在1.691~270μg·m L^(-1)(r=0.9995)、0.164~26.2μg·m L^(-1)(r=0.9995)、1.204~192μg·m L^(-1)(r=0.9995)、0.314~50.2μg·m L^(-1)(r=0.9995)、1.847~295μg·m L^(-1)(r=0.9995)、0.181~29.0μg·m L^(-1)(r=0.9995)与峰面积呈良好的线性关系;平均回收率(n=3)均大于97.7%。结论该方法简便、重复性好,为骨折合剂的综合质量评价提供了科学依据。
Objective To establish a method for the content determination of gallic acid, syringin, chlorogenic acid, caffeic acid, paeoniflorin and paeonol in Guzhe mixture by HPLC. Methods The separation was done on Hypersil ODS column （250 mm ×4.6 mm, 5 pro） by gradient elution with acetonitrile and 0.4% methanoic acid solution as the mobile phase at 1.0 mL · min^ - 1. The detection wavelength was 254 nm, the column temperature was 30 ℃ , and the injection volume was 5 μL. Results Gallic acid, syringin, chlorogenic acid, caffeic acid, paeoniflorin and paeonol had a good linearity at 1.691 -- 270 μg · mL ^- 1, 0.164 -- 26.2 μg · mL ^- 1, 1.204 -- 192μg · mL ^- 1, 0.314- 50.2μg · mL ^- 1, 1.847 -- 295 μg · mL ^- 1 and 0.181 -- 29.0μg · mL ^- 1, respec- tively. The average recoveries （n =3） were all more than 97.7%. Conclusion The method is simple and highly reproducible, which can be used for the quality control of Guzhe mixture.
出处
《中南药学》
CAS
2017年第2期210-213,共4页
Central South Pharmacy
基金
2012年度苏州市科技发展计划项目(编号:SYSD2012117)
