摘要
目的：建立基于化学计量学的综合质量评价方法,用于评价重楼药材的质量。方法：使用HPLC色谱仪,建立同时进行多指标含量测定和指纹图谱分析的测定法;采用液质联用推测指纹图谱中未知峰的可能结构,使用相似度分析、聚类热图分析和主成分分析等方法,从获得的数据分析重楼药材的质量。结果：鉴别了重楼指纹图谱的10个共有峰可能的结构,其中5个为偏诺皂苷,5个为薯蓣皂苷;化学计量学可以对10批样品进行区分和评价;10批样品的总皂苷含量为11.7~78.4 mg·g^-1,云南重楼的总偏诺皂苷比为14.9%~40.9%。结论：建立的综合质量评价法简便、专属,可用于重楼的质量评价。
Objective： To establish a new analytical method for the overall quality evaluation of medicinal materials Paridis Rhizoma by multiple components determination based on the chemometrics. Methods： First,method for multiple components determination and fingerprint identification were established with the same HPLC conditions. Second,the probable structures of fingerprint unknown diffraction peaks were presumed with LC-MS. Third,obtained data were analyzed with similarity analysis,hierarchical cluster analysis（ HCA）,and principal component analysis.Results： The probable structures of ten fingerprint common peaks were identified,including 5 pennogenins and 5 diosgenins. Ten Paridis Rhizoma samples were discriminated and evaluated by chemometrics. In these ten samples,the total saponin contents were 11. 7- 78. 4 mg·g^- 1,the ratio of total pennogenin contents and total saponin contents in Paris yunnanensis Franch. samples was 14. 9%- 40. 9%. Conclusion： It is feasible,convenient and exclusive to evaluate the overall quality of Paridis Rhizoma by determination of multiple components base on chemometrics.
出处
《药物分析杂志》
CAS
CSCD
北大核心
2015年第9期1585-1590,共6页
Chinese Journal of Pharmaceutical Analysis
