摘要
目的:探讨"形神共养"的丰富生存环境对正常大鼠认知功能、突触素(SYN)、微管相关蛋白-2(MAP-2)的表达及大脑m PFC区锥体细胞的影响。方法:健康雄性SD大鼠48只,随机分为"形养"组(EE1),"神养"组(EE2),"形神共养"组(EE3)和标准养组(SE),每组12只,分别进行形养、神养、形神共养的丰富生存环境训练及标准养。训练结束后进行水迷宫实验,水迷宫结束后用免疫组织化学方法检测海马区SYN、MAP-2的表达;用Golgi-Cox染色观察大脑内侧前额叶区锥体细胞树突的变化。结果:水迷宫测试的第5天,EE3组大鼠的潜伏期明显短于SE组(P<0.05);4组大鼠在目标象限停留的时间无明显差异(P>0.05)。SE组的SYN表达最低,而EE1组和EE3组之间的表达无明显差异且均高于EE2组。SE组的MAP-2表达最低,而EE3组的表达高于EE1组和EE2组。m PFC区锥体细胞树突交点总数4组组间比较差异无显著性意义(P>0.05)。棘密度EE3组最大,其次是EE1组,SE组与EE2组之间无区别且较EE1和EE3组低。结论:"形神共养"的丰富生存环境可提高正常大鼠的认知功能,并促进SYN及MAP-2免疫表达及锥体细胞树突生长。
Objective： To study the effect of the three kinds of EE on the cognitive function and the expression of SYN and MAP-2, and the dentritic growth of pyramidal cells of the cerebral cortex in rats. Method： Forty-eight male SD rats were divided into preserving the physique EE group （EEl）, preserving the spirit EE group （EE2）, preservation for both physique and spirit EE group（EE3） and standard group （SE）, 12 rats in each group. Rats were put into the three kinds of EE or standard cages after pre-training. The environmental enrollment lasted for 14 days and followed by a 6-days Morris water maze test. After the Morris water maze test, all the rats were sacrificed for histological study, including using the immunohistochemistry method to detect the expression of SYN and MAP-2, and using the Golgi-Cox staining to study the dentritic growth of pyramidal cells in cerebral cortex. Result： In the Morris water maze, the EE3 group exhibited shorter escape latency than the MCAO＋SE group （P〈0.05） on day 5, while no significant differences were seen for the time spent in the target quadrant among the 4 groups （P〉 0.05）. The results of immunohistochemical staining of SYN showed that the expression of SYN was the lowest in the SE group, and there was no significant difference between EEl and EE3 groups but significantly higher than the EE2 group. The expression of MAP-2 was the lowest in the SE group, and there was no significant difference of expression between EEl and EE2 groups but significantly lower than the EE3 group. The results of Golgi-Cox staining showed that there was no difference of the dentritic branch among groups in mPFC （P〉0.05） while the spine density of the EEl group was significantly greater than that of the SE and EE2 groups （P〈 0.05）, but significantly lower than that of the EE3 group （P〈 0.05）. Conclusion： Two weeks of ＂preservation from both physique and spirit＂ EE can improve the cognitive func- tion, and promote the expression of MAP-2 and SYN an
出处
《中国康复医学杂志》
CAS
CSCD
北大核心
2017年第4期384-390,共7页
Chinese Journal of Rehabilitation Medicine
基金
国家自然科学基金项目(81173315)
关键词
形神共养
丰富生存环境
突触素
微管相关蛋白-2
高尔基染色
preservation from both physique and spirit
enriched environment
synaptophysin
microtubule associ-ated protein-2
Golgi-Cox staining
