摘要
目的:观察microRNA-182在系统性红斑狼疮(systemic lupus erythematosus,SLE)患者外周血的表达水平,明确microRNA-182应用于SLE诊断的临床价值。方法:选取2014年1月至2015年1月在南京医科大学附属无锡市第二人民医院就诊的SLE患者80例(SLE组),同期招募健康体检者70例(对照组)。2组患者入院即刻抽取约5 mL静脉血,采用Taq Man real-time PCR方法测定所有受试者外周血microRNA-182表达水平。根据SLE患者是否首次发病,分为初发SLE亚组及非初发SLE亚组,并比较两亚组患者microRNA-182表达水平的差异。分析microRNA-182水平与抗ds-DNA抗体及SLE疾病活动度指数(systemic lupus erythematosus disease activity index,SLEDAI)的相关性。结果:SLE患者外周血中microRNA-182水平较健康对照组显著升高(1.58±0.69 vs.0.40±0.35,P<0.01),其中初发SLE患者microRNA-182水平较非初发SLE患者显著升高(2.05±0.56 vs.1.10±0.45,P<0.01),同时发现microRNA-182水平与SLEDAI(r=0.466,P<0.01)及抗ds-DNA抗体(r=0.442,P<0.01)具有显著相关性。同时,ROC曲线发现microRNA-182在诊断SLE方面具有较高价值[AUC:0.94(95%CI0.91~0.98)]。结论:microRNA-182可以作为诊断及SLE的生物标志物。
Objective：To study the expression of microRNA-182 in systemic lupus erythematosus（SLE） for exploring the value of diagnosis for SLE.Methods：Eighty SLE patients served as a SLE group and 70 healthy volunteers as a healthy control（HC） group from Wuxi Second People＇s Hospital of Nanjing Medical University between January 2014 and January 2015.About 5 mL venous blood was collected from all subjects on admission,and the levels of microRNA-182 were detected by Taq Man real-time（RT） PCR.Meanwhile,SLE patients were divided into a newly diagnosed subgroup（n=40） and a non-newly diagnosed subgroup（n=40）,the difference of expression microRNA-182 were compared in the two subgroups.The relationship between microRNA-182 levels and anti-ds-DNA antibody and SLEDAI were explored subsequently.Results：The levels of microRNA-182 in the SLE group were significantly higher than the HC group（1.58±0.69 vs.0.40±0.35,P〈0.01）,meanwhile its levels in newly diagnosed subgroup were significantly higher than non-newly diagnosed subgroup（2.05±0.56 vs.1.10±0.45,P〈0.01）.The expression of microRNA-182 had a positive correlation with anti-ds-DNA antibody and SLEDAI（r=0.466,P〈0.01 and r=0.442,P〈0.01,respectively）,and ROC suggested that the value of microRNA-182 in diagnosis for SLE was high [AUC：0.94（95% CI 0.91–0.98）].Conclusion：microRNA-182 is a potential biomarker in diagnosis for SLE.
作者
余伟
徐蔷薇
季蓉
黄红宇
任天丽
YU Wei XU Qjangwei JI Rong HUANG Hongyu REN Tianli(Department of Laboratory Medicine Department of Rheumatology, Wuxi Second People＇s Hospital of Nanjing Medical Universityj Wuxi Jiangsu 214002, China)
出处
《临床与病理杂志》
2017年第3期547-551,共5页
Journal of Clinical and Pathological Research
基金
无锡市医院管理中心医学科技项目(YGZXQ1301)~~
