摘要
目的:采用星点设计-效应面法,优选萆薢分清饮超声-微波协同提取工艺,并与传统水煎煮工艺进行对比。方法:分别考察微波频率、加水倍量、提取时间等主要因素,将薯蓣皂苷元、甘草酸提取率及浸膏收率作为评价指标,以综合评分为响应值作响应面和等高线,进行预测分析,获得最优提取条件。结果:确定萆薢分清饮的最优提取工艺:微波频率为434 W,加水倍量为18.4倍,提取时间为9.3 min,超声频率固定为50 W。采用该提取工艺,薯蓣皂苷元的提取收率为23.17%(mg·g^(-1)),甘草酸的提取收率为0.64%(g·g^(-1)),浸膏的提取收率为34.12%(g·g^(-1)),各项指标均高于传统水煎煮工艺。结论:星点设计-效应面法适用于萆薢分清饮的提取工艺优化,建立的数学模型预测性好,优化所得的超声-微波协同提取工艺操作简便,提取效率高,适用于工业化生产,同时也为萆薢分清饮的现代制剂研发提供科学理论基础。
This study aimed at optimizing the extraction process of Bi Xie Fen Qing（BXFQ） drink using ultrasoundmicrowave cooperation with central composite design-response surface method in comparison with the traditional decoction process. Taking microwave frequency, amount of water, extraction time as the main detection factors, diosgenin,glycyrrhizic acid and the extract yield were tested as the evaluation indexes; and taking an integrated score as response value for response surface and contour, predictive analysis was carried out and the optimum extraction conditions were achieved. It was found that the optimum extraction process of BXFQ drink was identified： the microwave frequency was434 W, water addition was 18.4 times, extraction time was 9.3 mins and the ultrasonic frequency was fixed at 50 W.Under the optimum process, the diosgenin extraction yield rate was 23.17%（mg·g-1）, extraction yield rate of glycyrrhizic acid was 0.64%（g·g-1）, and the extraction yield rate of extractum was 34.12%（g·g-1）. All the indexes were superior to those of the traditional method. It is concluded that the composite design-response surface method is suitable for the extraction optimization of BXFQ drink with favorable predictability of the mathematical model. The optimized ultrasoundmicrowave cooperation was easy to operate with high extraction efficiency. It is suitable for industrialized production with the provision of a scientific reference for the modern formulation development of BXFQ drink.
出处
《世界科学技术-中医药现代化》
CSCD
2017年第2期351-358,共8页
Modernization of Traditional Chinese Medicine and Materia Medica-World Science and Technology
基金
江西省教育厅科学技术研究项目(GJJ151340):中药复方温敏凝胶直肠给药系统的研究
负责人:叶颖俊
关键词
萆薢分清
超声-微波协同
星点设计
提取率
Bi Xie Fen Qing drink
ultrasound-microwave cooperation
central composite design
extraction rate
