摘要
为了明确玉米C_4型pepc(磷酸烯醇式丙酮酸羧化酶基因)、ppdk(丙酮酸磷酸双激酶基因)、nadp-me(NADP-苹果酸酶基因)基因对拟南芥光合特性的影响效应,以PC(转Zm pepc基因)、PK(转Zm ppdk基因)、ME(转Zm nadp-me基因)、PCK(转Zm pepc+ppdk基因)、PKM(转Zm ppdk+nadp-me基因)5种转基因拟南芥和野生型WT为材料,鉴定了5种转基因拟南芥株系的外源基因表达量,测定了250μmol·m^(-2)·s^(-1)、800μmol·m^(-2)·s^(-1)、1 400μmol·m^(-2)·s^(-1)3种光照强度处理下转基因拟南芥株系相应的光合酶活性、光合速率。结果表明,5种转基因拟南芥在转录、翻译水平上均高表达了导入的外源基因。250μmol·m^(-2)·s^(-1)光强下,转基因拟南芥材料PC、PK、ME、PCK、PKM的PEPC酶活性较WT分别提高14.4%、9.1%、4.4%、21.6%、3.5%;PPDK酶活性较WT分别提高5.2%、33.2%、14.8%、24.9%、8.1%;NADP-ME酶活性较WT分别提高12.8%、42.0%、126.4%、31.9%、64.6%;净光合速率较WT分别提高26.6%、26.4%、5.4%、43.1%、14.1%,其中PC、PCK的C_4光合酶活性和净光合速率表现最为突出。随着光强胁迫的增加,转基因拟南芥上述测定指标较WT高出的幅度增加,较250μmol·m^(-2)·s^(-1)光强下降低的幅度小于WT,说明转基因拟南芥耐强光胁迫能力更强。转基因拟南芥酶活特性、光合速率存在基因型差异,单基因材料PC>PK>ME,双基因材料PCK>PKM,且PCK为本研究所有基因型中最优的材料。
To clear the effects of maize C_4-type Zmpepc（phosphoenopyruvate carboxylase）, Zmppdk（pyruratedikinase） and Zmnadp-me（NADP-malic enzyme） genes on photosynthetic characteristics of Arabidopsis thaliana. Transgenic A. thaliana PC（overexpressing Zmpepc）, PK（overexpressing Zmppdk）, ME（overexpressing Zmnadp-me）,PCK（overexpressing Zmpepc＋Zmppdk）, PKM（overexpressing Zmppdk＋Zmnadp-me） and wild type A. thaliana were used as experimental materials. We identified the relative expressions of these exogenous genes in 5 transgenic Arabidopsis lines under normal condition and measured the corresponding photosynthetic enzyme activities and photosynthetic rates of different transgenic A. thaliana under light intensities of 250 μmol·m^（-2）·s^（-1）, 800 μmol·m^（-2）·s^（-1）and 1 400 μmol·m^（-2）·s^（-1）. The results showed that the exogenous genes were highly expressed at the transcription and translation levels in transgenic A. thaliana. Under 250 μmol·m^（-2）·s^（-1）light intensity, compared to the WT,PEPC enzyme activity in transgenic A. thaliana PC, PK, ME, PCK and PKM was increased by 14.4%, 9.1%, 4.4%,21.6% and 3.5%, respectively; PPDK enzyme activity was increased by 5.2%, 33.2%, 14.8%, 24.9% and 8.1%,respectively; NADP-ME enzyme activity was increased by 12.8 %, 42.0 %, 126.4 %, 31.9 % and 64.6%,respectively; and Pn was increased by 26.6 %, 26.4 %, 5.4 %, 43.1 % and 14.1%, respectively. The C_4 photosynthetic enzyme activity and photosynthetic rate of PC and PCK had the largest increase in these parameters compared to these with other transgenes. With the increasing of light stress intensity, the above measured parameters in the transgenic A. thaliana was larger than in WT, and the degree of decrease was lower than in WT in 250 μmol·m^（-2）·s^（-1）light intensity, indicating transgenic A. thaliana had a higher level of tolerance to strong light stress. Thus, the enzyme activity characteristics and photosynthetic rate of transgenic Arabidopsis
出处
《分子植物育种》
CAS
CSCD
北大核心
2017年第3期911-919,共9页
Molecular Plant Breeding
基金
国家自然科学基金项目(31371707)
国家转基因生物新品种培育科技重大专项(2016ZX08002-003)
河南省重大科技专项(151100111400)
河南省基础研究项目(132300410005
132300410283)共同资助
关键词
拟南芥
C4关键酶基因
光强
光合特性
Arabidopsis thaliana
C_4 key enzymes gene
Light intensity
Photosynthetic characteristics
