摘要
通过利用新一代高通量测序手段——转录组测序(RNA-Seq)技术研究巴西蕉(Musa paradisiaca)叶片在60 mmol/L NaCl人工模拟盐胁迫0、12 h、24 h不同时间点的转录组分析。结果表明:盐胁迫12 h上调和下调的DEGs(differential-expressed genes)分别为2 938个和2 727个;24 h分别有834个和893个。GO富集分析,差异基因主要在细胞过程、代谢过程、刺激响应、结合以及催化活性等。KEGG分析,差异基因主要涉及代谢途径、光合作用、黄酮类生物合成、苯丙素生物合成等。通过GO和KEGG显著性富集分析发现,NaCl胁迫12 h后差异表达的基因数明显多于24 h胁迫后的差异基因表达数。qRT-PCR验证了DEGs的表达趋势与RNA-Sep测序分析结果的一致,证明了RNA-Sep结果的准确性。本研究通过香蕉叶片转录组分析,为研究香蕉耐盐分子机制提供参考。
In this research, through the next-generation high-throughput sequencing technology-RNA-seq, to study artificial simulated salt stress environment at 60 mmol/L NaCl, transcriptome analysis the leaves of Brazil banana（Musa paradisiaca） under NaCl stress 0, 12 h, 24 h at different times. The results showed that： compared with the control, there were 2 938 and 2 727 differentially expressed genes（DEGs） up-and down-regulated respectively coercion 12 h; coercion 24 h, there were 834 genes up-regulated, 893 genes down-regulated. GO enrichment analysis showed these DEGs were involved in pathways like cellular processes, metabolic processes, response to stimulus,binding, and catalytic activity and so on. KEGG pathway analysis showed that these DEGs were involved in metabolic pathways, photosynthesis, phenylpropanoid biosynthesis and flavonoid biosynthesis. By GO and KEGG significant enrichment analysis found that： after 12 h NaCl stress the number of differentially expressed genes was significantly higher than after 24 h salt stress. The results of qRT-PCR confirmed that the expression of DEGs was consistent with the result of RNA-Sep sequencing analysis, which proved the accuracy of RNA-Sep. This research through studied transcriptome analysis of banana leaves, which provided a reference for studying the molecular mechanism of banana salt tolerance.
出处
《分子植物育种》
CAS
CSCD
北大核心
2017年第3期875-882,共8页
Molecular Plant Breeding
基金
国家自然科学基金项目(31260462)资助
关键词
巴西蕉
转录组
盐胁迫
Musa paradisiaca
Transcriptome
Salt stess
