摘要
以姜黄组培苗为试验材料，在继代培养基中分别添加0、25、50、75、100、125、150mmol·L^-1NaCl进行盐胁迫处理，研究NaCl胁迫下姜黄幼苗生长和生理特性的变化。结果表明：NaCl胁迫下，姜黄组培苗的脯氨酸和丙二醛含量逐渐上升，而叶绿素和类胡萝卜素含量、超氧化物歧化酶（SOD）和过氧化氢酶（CAT）活性逐渐下降。25mmol·L^-1NaCl时姜黄幼苗的基径、株高和鲜重与对照差异不显著，〉50mmol·L^-1NaCl时则显著下降；表明姜黄组培苗的生长可以耐受25～50mmol·L^-1NaCl胁迫。随着NaCI浓度增加，姜黄幼苗的可溶性糖和蛋白质含量、过氧化物酶（POD）活性先升高后降低，75mmol·L^-1NaCI时均达到最大值。50～100mmol·L^-1。NaCl胁迫下姜黄叶片的苯丙氨酸解氨酶（PAL）活性和姜黄素含量得到显著提高，表明50～100mmol·L^-1NaCl胁迫对姜黄素的合成有促进作用。可见．25～50mmol·L—NaCl能够促进姜黄植株的生长，而75mmol·L^-1NaCl显著提高次生代谢产物姜黄素的含量。
A tissue culture experiment was conducted to investigate the changes of the growth, physiological characteristics and curcumin concentration of in vitro Curcuma aromatica plantlets under salt stress （0, 25, 50, 75, 100, 125 or 150 mmol·L^-1 of NaC1）. Results showed that the concentrations of proline and malondialdedyde （MDA） of in vitro C. aromatica plantlets in- creased, while the concentrations of chlorophyll and carotenoid and the activities of superoxide dismutase （SOD） and catalase （CAT） decreased significantly under salt stress. No significant differences in the basal diameter, height and fresh weight were observed between the 25 mmol·L^-1 NaC1 and control treatments, while those significantly decreased under over 50 mmol·L^-1 NaCl, suggesting that the plantlets of C. aromatica could tolerate 25-50 mmol ·L^-1 NaC1 stress. With the stress increasing, the soluble sugar concentration and peroxidase （POD） activity in C. aromatica increased in the early phase of stress and decreased subsequently, and the maxi- mum was present under 75 mmol ·L^-1 NaC1 stress. The phenylalanine ammonia lyase （PAL） ac- tivity and curcumin concentration in leaves significantly improved under 50-100 mmol ·L^-1 NaCl treatment, indicating that this salt stress promoted the formation of curcumin. Therefore, the range of 25-50 mmol ·L^-1NaCI benefits the growth of C. aromatica plantlets, while the 75 mmol ·L^-1 NaCl increases the curcumin concentration significantly.
出处
《生态学杂志》
CAS
CSCD
北大核心
2014年第2期388-393,共6页
Chinese Journal of Ecology
基金
国家自然科学基金项目(31101512)
华侨大学基本科研业务费专项基金(JB-ZR1151)资助
关键词
盐胁迫
姜黄
组培苗
姜黄素
生理效应
salt stress
Curcuma aromatica
tissue culture seedlings
curcumin
physiologicaleffects.
