摘要
为了进一步探讨经SSH筛选的蒙古羊繁殖差异基因BMP6对蒙古羊双羔性状的影响及作用机制,利用实时荧光定量PCR技术(RT-qPCR)检测了单、双羔蒙古羊发情第0、5、10、15天时卵巢组织中BMP6基因的表达水平,比较了BMP6基因在蒙古羊单、双羔群体中的差异表达情况。结果表明,在一个发情周期中,单羔组和双羔组蒙古羊卵巢组织中BMP6基因的mRNA表达水平变化趋势相似:发情第0天表达量最低,发情第10天时表达量最高,且显著高于其他时期(P<0.05);双羔组发情第0天和第10天的BMP6基因mRNA表达量分别显著(P<0.05)和极显著(P<0.01)高于相应时期的单羔组。综上提示,BMP6基因是影响蒙古羊双羔性状的候选基因。
This study aimed to assess the effect of a differentially expressed gene BMP6 screened by SSH on twining trait of Mongolian Sheep and its underling mechanism. The mRNA expression pattern of BMP6 gene in ovarian tissues of multiparous and uniparous Mongolian ewes on the 0^th,5^th,10^ th,15^ thday of oestrum were determined and statistically compared by RT-qPCR. The results showed that during a whole estrous cycle,the mRNA expression profile of BMP6 gene in ovarian tissues of multiparous and uniparous Mongolian ewes exhibited a similar trend of change： on the 0thday of oestrum,the lowest expression level of BMP6 gene was observed,and the highest expression level was observed on the 10 ^thday of oestrum（P〈0.05）; on the 0^thand 10^ thday of oestrum,the mRNA expression of BMP6 gene in multiparous Mongolian ewes was significantly higher（P〈0.05） and very significantly higher（P〈0.01） than that of single-lamb Mongolian ewes,respectively. The obtained results demonstrate that BMP6 is a candidate gene for twining trait of Mongolian Sheep.
出处
《畜牧与饲料科学》
2017年第3期25-27,31,共4页
Animal Husbandry and Feed Science
基金
内蒙古农牧业科学院青年基金项目(2013QNJJM06)
