摘要
根据GenBank发表的绵羊骨形态发生蛋白6(bone morphogenetic protein 6,BMP6)基因部分序列所包含的外显子5、6、7和小鼠BMP6基因外显子5、6、7序列设计3对引物,采用PCR-SSCP技术检测BMP6基因外显子5、6和7在济宁青山羊、安哥拉山羊、波尔山羊、内蒙古绒山羊4个山羊品种250个个体中的单核苷酸多态性。结果发现这3对引物的扩增片段在检测的4个品种中均无多态性,说明所检测的BMP6基因外显子5、6、7序列比较保守。
Single nucleotide polymorphisms (SNPs) of exons 5, 6, 7 of bone morphogenetic protein 6 (BMP6) gene were detected in four goat breeds (Jining Grey, Angora, Boer and Inner Mongolia Cashmere goats) with three pairs of primers by polymerase chain reaction (PCR)-single strand conformation polymorphism (SSCP). No polymorphisms were detected in the amplified regions of three pairs of primers in four goat breeds tested, which may indicate that the sequences of exons 5, 6, 7 of BMP6 gene were rather conserved.
出处
《中国畜牧兽医》
CAS
2008年第1期55-58,共4页
China Animal Husbandry & Veterinary Medicine
基金
国家973计划项目(2006CB102105)
国家863计划项目(2006AA10Z139)
国家自然科学基金项目(30540052)
北京市自然科学基金项目(6062023)资助