摘要
Controlled release of the functional factors is the key to improve clinical therapeutic efficacy during the tissue repair and regeneration. The thrce-dimensional （3D） scaffold can provide not only physical properties such as high strength and porosity hut also an optimal environment to enhance tissue regeneration. Sphingosine 1-phosphate （SIP）, an angiogenlc factor, was loaded into mesoporous silica nanoparticles （MSNs） and then incorporated into poly （ L-lactic add ） （ PLLA ） nanofibrons scaffold, which was fabricated by thermally induced phase separation （TIPS） method. The prepared scaffolds were examined by attenuated total reflection Fourier transform infrared spectroscopy （ATR-FTIR）, scanning electron microscopy （ SEM）, and transmission electron microscopy （TEM） and compressive mechanical test. The ATR-FTIR result demonstrated the existence of MSNs in the PLLA nanofibrous scaffold. The SEM images showed that PLLA scaffold had regular pore channel, interconnected pores and nanofibrous structure. The addition of MSNs at appropriate content had no visible effect on the structure of scaffold. The compressive modulus of scaffold containing MSNs was higher than that of the scaffold without MSNs. Furthermore, fluorescein isothiocyanate （FTTC） was used as model molecule to investigate the release behavior of SIP from MSNs- incorporated PLLA （MSNs/PLLA） nanofibrons scaffold. The result showed that the composite scaffold largely reduced the initial burst release and exhibited prolonged release of FITC than MSNs. Thus, these results indicated that SIP-loaded composite uanofibrons scaffold has potential applications for bone tissue engneering.
Controlled release of the functional factors is the key to improve clinical therapeutic efficacy during the tissue repair and regeneration.The three-dimensional(3D)scaffold can provide not only physical properties such as high strength and porosity but also an optimal environment to enhance tissue regeneration.Sphingosine1-phosphate(S1P),an angiogenic factor,was loaded into mesoporous silica nanoparticles(MSNs)and then incorporated into poly(L-lactic acid)(PLLA)nanofibrous scaffold,which was fabricated by thermally induced phase separation(TIPS)method.The prepared scaffolds were examined by attenuated total reflection Fourier transform infrared spectroscopy(ATR-FTIR),scanning electron microscopy(SEM),and transmission electron microscopy(TEM)and compressive mechanical test.The ATR-FTIR result demonstrated the existence of MSNs in the PLLA nanofibrous scaffold.The SEM images showed that PLLA scaffold had regular pore channel,interconnected pores and nanofibrous structure.The addition of MSNs at appropriate content had no visible effect on the structure of scaffold.The compressive modulus of scaffold containing MSNs was higher than that of the scaffold without MSNs.Furthermore,fluorescein isothiocyanate(FITC)was used as model molecule to investigate the release behavior of S1P from MSNsincorporated PLLA(MSNs/PLLA)nanofibrous scaffold.The result showed that the composite scaffold largely reduced the initial burst release and exhibited prolonged release of FITC than MSNs.Thus,these results indicated that S1P-loaded composite nanofibrous scaffold has potential applications for bone tissue engineering.
基金
National Natural Science Foundations of China(Nos.31271028,31570984)
International Cooperation Fund of the Science and Technology Commission of Shanghai Municipality,China(No.15540723400)
Open Foundation of State Key Laboratory for Modification of Chemical Fibers,Polymer Materials,China(No.LK1416)
the Innovation Funds of Donghua University,China(No.15D310516)
“111 Project” Biomedical Textile Materials Science and Technology,China(No.B07024)
