摘要
目的探讨聚左旋丙交酯[Poly(l-lactic acid),PLLA]纳米纤维支架与大鼠脊髓组织的相容性。方法以PLLA为原料,采用液-液相分离技术构建纳米纤维支架(Nanofibrous scaffolds)。在体外构建骨髓间充质干细胞(MSCs)-PLLA纳米纤维支架复合体;将12只SD大鼠随机分为两组,即损伤对照组和MSCs移植组。暴露大鼠胸10脊髓段做半横断损伤,其中在损伤对照组脊髓损伤处移植PLLA纳米纤维支架,在MSCs移植组脊髓损伤处移植MSCs-PLLA纳米纤维支架复合体。术后两组动物观察60d。HE染色观察结构改变,免疫组织化学方法观察炎症细胞及MSCs的存活情况。结果体外成功构建MSCs-PLLA纳米纤维支架复合体。扫描电镜显示,PLLA纳米纤维支架由相互贯穿的微孔组成。荧光显微镜和扫描电镜下均可见MSCs能够很好地黏附在支架上,分布较均匀。移植术后60d,HE染色显示,PLLA纳米纤维支架与脊髓组织融合较好,无明显空洞和瘢痕。CD68免疫组织化学显示,在移植物与宿主脊髓交界处有少量阳性细胞,表明有轻微炎症反应。GFP免疫组织化学显示,移植在脊髓损伤处的PLLA纳米纤维支架内有大量阳性MSCs,表明MSCs在移植术60d仍有存活。结论PLLA纳米纤维支架与大鼠脊髓组织有较好的生物相容性。
Objective To explore the biocompatibility of the Poly （l-lactic acid） （PLLA） nano-flbrous scaffold with spinal cord tissue of rats, Methods PLLA three-dimensional （3D） scaffolds with macro/micro-pores and nano-fibrous structure were fabricated by phase separation from a ternary PLLA/dioxane/water system. MSCs-PLLA nano-fibrous scaffolds complex were constructed in vitro. Twelve SD rats were divided into two groups： control injured group and MSCs transplanted group. T10 spinal cord segment was exposed, and then transected. About 2 mm spinal cord was removed. PLLA nano-fibrous scaffolds were transplanted into the transected site of spinal cord in the control injured group. MSCs-PLLA complex were transplanted into the injured site in the MSCs transplanted group. Sixty days after operation, the histological observations and immunohistochemistry were carried out. Results PLLA nano-fibrous scaffolds interconnected micropores were composed. The well-distributed MSCs in MSCs-PLLA nano-fibrous scaffold complex can be observed under the scanning electron microscope and fluorescence microscope. Sixty days after transplantion, there were no scars and big cavitates between spinal cord and PLLA nano-fibrous scaffold. There was no obvious inflammatory response under the CD68 immunohistochemistry staining between the host spinal cord and transplantion. Sixty days after transplantation, there were large amount of MSCs in the PLLA nano-fibrous scaffold under GFP immunohistochemistry staining. Conclusion The PLLA nano-fibrous scaffolds is a good matrix, which has good biocompatibility rat spinal cord tissue in rats.
出处
《组织工程与重建外科杂志》
2009年第3期143-147,共5页
Journal of Tissue Engineering and Reconstructive Surgery
基金
国家自然科学基金(30771143)
广东省自然科学基金(07117373)
