摘要
目的:观察小半夏汤对化疗呕吐模型大鼠膈下迷走神经放电的影响,探讨小半夏汤的止吐机制。方法:雄性SD大鼠分为空白组、模型组、昂丹司琼治疗组(2.6 mg·kg^(-1))、阿瑞匹坦治疗组(11.08 mg·kg^(-1))、小半夏汤大中小剂量治疗组(3.2 g·kg^(-1)、1.6 g·kg^(-1)、0.8 g·kg^(-1))、小半夏汤正常对照组(1.6 g·kg^(-1))。造模前3 d开始给药,上述剂量每天分2次给予(间隔12 h),空白组、模型组灌胃等容积蒸馏水。第三天第5次灌胃1 h后,麻醉动物,颈静脉分别注射顺铂、1-苯基双缩胍(1-PBG)和P物质建立大鼠化疗性呕吐模型,采用电生理方法记录注射上述3种工具药前后大鼠膈下迷走神经放电幅度变化。结果:分别注射上述3种工具药后,与空白组比较,模型组大鼠膈下迷走神经各时间点放电活动均显著增强(P<0.05,P<0.01,P<0.001);与模型组比较,小半夏汤大、中剂量均可显著抑制上述工具药所致的膈下迷走神经放电增强(各时间点均P<0.001)。结论:小半夏汤可显著抑制顺铂、1-PBG和P物质所致的大鼠膈下迷走神经放电活动增强,提示小半夏汤通过阻断膈下迷走神经末梢上的5-羟色胺3(5-HT_3)受体和神经激肽1(NK_1)受体,阻断了迷走神经传入纤维将呕吐信号传至呕吐中枢,从而发挥防治化疗性恶心呕吐作用。
Objective：To observe the effects of Xiaobanxia decoction（XBXD） on the discharge of subdiaphragmatic vagal nerve in chemotherapy-induced vomiting model rats in order to explore the antiemetic mechanism of XBXD.Methods：Male SD rats were divided into the normal control group,model group,ondansetron treatment group（2.6 mg·kg^-1）,aprepitant treatment group（11.08 mg·kg^-1）,XBXD high,middle and small dose treatment groups（3.2 g·kg^-1,1.6 g·kg^-1,0.8 g·kg^-1）,XBXD normal control group（1.6 g·kg^-1）.XBXD and positive medicine were gavaged 3 days before the injection of tool drug and lasted for 3 days,the normal group and model group were gavaged with same volume of distilled water.After the last administration,rats were anesthetized,cisplatin,1-phenylbiguanide（1-PBG） or substances P were injected by jugular vein respectively to establish the chemotherapy-induced vomiting model.The discharge amplitude changes of the subdiaphragmatic vagal nerve were recorded by the electrophysiological methods before and after the injection of these tool drugs.Results：After injecting the tool drugs,the discharge of subdiaphragmatic vagal nerve of the model group was significantly enhanced（compared with normal group at each time point,P〈0.001）;XBXD high and middle dose could significantly inhibit the increase of subdiaphragmatic vagal nerve discharge（compared with model group at each time point,P〈0.001）.Conclusion：XBXD can significantly inhibit the discharge of subdiaphragmatic vagal nerve activity induced by cisplatin,1-PBG and substance P respectively.The present results implied that XBXD could block 5-HT3 receptor and NK1 receptor in subdiaphragmatic vagal nerve,and inhibited impulse from vagal nerve transferred to vomiting center,which could be a underlying mechanism of XBXD to prevent and treat chemotherapy-induced nausea and vomiting.
出处
《山东中医药大学学报》
2017年第2期170-173,共4页
Journal of Shandong University of Traditional Chinese Medicine
基金
国家自然科学基金面上项目(编号:81373828)
关键词
小半夏汤
膈下迷走神经放电
顺铂
P物质
1-苯基双缩胍
止吐机制
大鼠
Xiaobanxia decoction
subdiaphragmatic vagal nerve discharge
cisplatin
substance P
1-phenylbiguanide
antiemetic mechanism
rats
