摘要
采用佛波酯(PMA)致小鼠耳廓肿胀模型,以丙酮为空白组(阴性对照组),PMA为对照组(阳性对照组),考察了赶黄草的抗炎性能,研究发现不同浓度的赶黄草提取物对PMA致小鼠耳廓肿胀度均有不同程度的抑制作用,且抑制作用呈剂量依赖性,当赶黄草的浓度为10 mg/15μL时,其抑制肿胀效果达到35.4%;HE染色研究结果表明,赶黄草可有效抑制小鼠耳朵炎症;通过免疫组化法研究赶黄草对IL-1β,IL-6,TNF-α的表达及活性的影响,研究赶黄草的抗炎机制。结果显示与PMA对照组比较,赶黄草提取物可显著抑制IL-1β、IL-6、TNF-α等致炎因子的表达(P<0.05)。
To study the anti-inflammatory activities and mechanism of Penthorum chinense Pursh,phorbol-12-myris- tate13-acetate （PMA）- induced mice ear edema model is adopted with Acetone as the blank group （negative con- trol） and PMA as the control group （ positive control）. The results show that Penthorum chinense Pursh extractive of varying concentration could obviously reduce the swelling of mouse ear edema in a different degree and dose-de- pendent way. The inhibitory rate reaches 35.4% when the concentration is 10 mg/15 L. The study on HE also re- veals that Penthorum chinense can effectively inhibit mice ear inflammation. By analyzing the anti-inflammatory mechanism of Penthorum chinense Pursh from its influence on the presentation and activity of IL-1β, IL-6 and TNF- α through immunohistochemistry method,it is found that in comparison with the PMA Control group, Pursh Pentho- rum chinense Pursh can effectively inhibit the presentation of inflammatory agents like IL-1β,IL-6 ,TNF-α,etc.
出处
《西华师范大学学报（自然科学版）》
2017年第1期48-51,74,共5页
Journal of China West Normal University(Natural Sciences)
基金
国家自然科学基金项目(21277109)
四川省科技厅应用基础研究项目(2015JY0254)
四川省教育厅重点项目(15ZA0320)
关键词
赶黄草
佛波酯
抗炎
Penthorum chinense Pursh
Phorbol-12-myristate13-acetate （PMA）
Anti-inflammatory
