摘要
目的分析滨州地区供血者ABO亚型的分子机制、分布情况。方法采用试管法对微板法筛查出的正反定型不符血样做进一步血型血清学分析,并进行亚型确认。对亚型标本进行血清学分析和DNA测序,测序结果用软件比对分析后,确定其基因型。结果从2014~2015年滨州地区无偿献血者84 394份血样中检出ABO亚型12例(检出率为1.4/万),其中A亚型3例(A^(el)1例、A_31例、A^(el)B1例)占25%,B亚型8例(B^(el)1例、Bx1例、B_32例、AB_34例)占66.7%,B(A)表型1例占8.3%。对12例亚型标本进行DNA测序结果显示,Ael05基因2例、B301基因1例、B303基因4例、B(A)04基因1例,但有A亚型1例、B亚型3例,6、7外显子测序未见明显变化。结论 ABO亚型分布存在不均衡性和地域性差异,滨州地区最常见的ABO亚型为AB3或B3,最常见突变基因为突变发生在第3内含子的B303基因。
Objective To analyze the molecular mechanism and distribution of ABO subtypes in Binzhou area.Methods The test tube method was used to do further serological analysis and subtype confirmation of the positive and negative serotypes of the blood samples selected by the micro plate. Serological analysis and DNA sequencing of the subtype samples were conducted. The genotype was determined after comparing and analyzing the sequencing results using the software. Results Twelve cases of ABO subtype were detected from the 84 394 blood samples from volunteer blood donors（ at a detection rate of 1. 4/10 000）,among which,three cases with A subtypes（ 1 case of A^（el）,1 case of A3,1 case of AelB） accounted for 25%,eight cases with B subtypes（ 1 case of Bel,1 case of Bx,2 cases of B3,4 cases of AB3） accounted for 66. 7%,one case in B（ A） type accounted for 8. 3%. From DNA sequencing,results on 12 samples showed that there were 2 cases of Ael05 gene,1 case of B301 gene,4 cases of B303 gene,1 case of B（ A） 04 gene. But 1 case in A subtype and 3 cases in B subtype. No significant changes were found in exon 6 and exon 7.Conclusion The distribution of ABO subtypes is unbalanced and shows regional differences. The most common subtype of ABO in Binzhou is AB3 or B3. The most frequently mutated gene is the B303 gene of the third intron.
出处
《中国输血杂志》
CAS
北大核心
2017年第2期135-138,共4页
Chinese Journal of Blood Transfusion
关键词
滨州地区
ABO血型
亚型
Binzhou region
ABO blood type
subtype
