摘要
目的评价标本溶血对酮胺氧化酶法测定糖化清蛋白(GA)的影响,并建立有效的校正方法。方法酮胺氧化酶法测定无溶血血清GA浓度及其对应人工溶血血清GA浓度、溶血指数;分析溶血对GA检测结果的影响及二者的相关性;利用多元回归分析建立纠正公式,用于临床溶血标本的GA测定值的数学纠正。结果溶血降低酮胺氧化酶法GA测定结果(P<0.01),溶血程度与GA浓度呈负相关(R^2=0.943 4);以无溶血血清GA浓度为Y,其对应人工溶血血清GA浓度为Z,溶血吸光度值为X,经多元回归分析,纠正公式为Y=2.468X+Z-0.015 73;溶血标本GA浓度经公式纠正后GA浓度偏移均小于10%。结论标本溶血可导致酮胺氧化酶法GA检测结果降低,运用纠正公式可有效校正溶血对GA测定的干扰,符合临床要求。
Objective To investigate the effects of hemolysis on glycated albumin（ GA） determined by ketoamine oxidase method and its correction. Methods GA concentration and hemolytic parameter（ optical density,A） in non-hemolytic serum and different degree hemolytic serum samples were measured. The impact of hemolysis on GA and the relationship between hemolysis and GA were analysed. A formula was developed to correct the interference of hemolysis on GA measurement using regressive Multiple analysis. Results Compared with non-hemolytic serum,hemolysis resulted in the significantly decreased concentrations of GA detected by ketoamine oxidase method（ P〈0. 01）,which were significantly associated with the degree of hemolysis（ R2= 0. 943 4）. Y and Z represented GA concentration of non-hemolytic serum and different degree hemolytic serum,while X represented optical density of hemolytic parameter. Formulas for GA measurement were presented： Y = 2. 468 X ＋ Z-0. 015 73,GA concentrations of hemolytic samples can be reverted to the values without statistical difference from the GA concentration in corresponding non-hemolytic samples. The bias of corrected GA was less than 10%.Conclusion Our results indicate that the level of GA measured through ketoamine oxidase method is negatively affected by hemolysis.The formula of mathematical correction of GA results in hemolytic samples should be suitable for the requirements of clinical laboratory.
出处
《临床检验杂志》
CAS
CSCD
2017年第2期94-97,共4页
Chinese Journal of Clinical Laboratory Science
基金
国家自然科学基金面上项目(81671836)
关键词
溶血
糖化清蛋白
干扰
校正
hemolysis
glycated albumin
interference
correction
