摘要
目的了解近4年来武警上海市总队医院男性不育患者精液中支原体检测阳性率和耐药情况,为临床抗菌药物的合理使用提供参考。方法以4年来的3 278例男性不育患者和348例健康对照者为研究对象,采用固体-液体培养基联合检测对精液标本中的脲原体属和人型支原体进行培养、鉴定和药敏试验;采用SPSS 21.0软件进行比较分析。结果近4年男性不育患者精液脲原体属和人型支原体的总阳性率为35%-50%,以脲原体属为主,其阳性率为31%-40%,均显著高于健康对照组（P〈0.05）;2012-2014年各种支原体阳性率变化不大;2015年总阳性率和脲原体属的阳性率高于前几年的阳性率,差异有统计学意义（P〈0.05）;1 259例男性不育者支原体阳性的年龄主要集中在26-30岁和31-35岁年龄段,分别为37.8%和32.1%。在10种抗菌药物中,脲原体属对四环素类和大环内酯类抗菌药物的耐药率较低,均小于5%,4年中对各种药物的耐药率变化不大。结论该地区近4年来男性不育患者精液中支原体阳性率较高,且以脲原体属为主,多集中于26-30岁和31-35岁年龄段,与前3年相比,2015年阳性率明显升高,近4年来各种药物的耐药性变化不大,治疗上应首选四环素类和大环内酯类抗菌药物。
Objective This study was undertaken to investigate the prevalence and antibiotic resistance profile of Mycoplasma in semen samples from infertile males during the period from 2012 to 2015 in Shanghai.Methods A total of 3 278 infertile male patients and 348 healthy controls were recruited into the study from the infertility clinic from 2012 to 2015 in our hospital.Culture,identification and the antimicrobial susceptibility testing were done to characterize Ureaplasma and Mycoplasma hominis strains.SPSS 21.0 software was used to analyze the data.Results In 3 278 cases of infertile men,the prevalence of Mycoplasma was35%-50%from 2012 to 2015.The prevalence of Ureaplasma strains was predominant from 31%to 40%,significantly higher than in healthy controls（P〈0.05）.The prevalence of total Mycoplasma and Ureaplasma in infertile males was significantly higher in2015 than in other 4 years（P〈0.05）.The males of 26 to 30 and 31 to 35 years of age showed high risk of Mycoplasma infection in the 1 259 infertile males,evidenced by positive Mycoplasma in 37.8%and 32.1%,respectively.The Ureaplasma strains showed the lowest resistance rate to semisynthetic tetracyclines and macrolides（〈5% resistant）.No obvious change about the resistant rates was observed in the last 4 years.Conclusions The prevalence of Mycoplasma,especially Ureaplasma spp.,is high in the infertile males in Shanghai.The prevalence of Mycoplasma in 2015 was the highest in the last 4 years.The males of 26 to30 and 31 to 35 years of age have high risk of Mycoplasma infections in the infertile males.Tetracyclines and macrolides are of choice for the potential Mycoplasma infection in the infertile males in Shanghai.
出处
《中国感染与化疗杂志》
CAS
CSCD
北大核心
2016年第5期648-652,共5页
Chinese Journal of Infection and Chemotherapy
基金
上海市自然科学基金(13ZR1449700)
关键词
脲原体属
人型支原体
不育
耐药性
Ureaplasma spp
Mycoplasma hominis
infertility
antimicrobial susceptibility testing
