摘要
目的:观察表没食子儿茶素没食子酸酯(epigallocatechin-3-gallate,EGCG)对甲状腺癌细胞增殖和摄碘能力的影响。方法:用不同浓度的EGCG处理甲状腺癌W3细胞株48 h后,用3-(4,5-二甲基噻唑-2)-2,5-二苯基四氮唑溴盐(MTT)法检测W3细胞增殖能力,流式细胞术、免疫荧光法检测钠-碘转运体(sodium iodide symporter,NIS)的变化,摄碘能力实验检测W3细胞摄碘能力。结果:与对照组比较,随着EGCG浓度的增加,W3细胞的增殖能力逐渐降低(P<0.01),NIS的表达逐渐增加(P<0.01),呈浓度依赖性。摄碘实验显示,在一定浓度范围内,EGCG明显促进W3细胞对碘的吸收能力(P<0.01)。结论:EGCG能抑制甲状腺癌细胞的增殖能力,通过上调NIS的表达促进甲状腺癌细胞株的摄碘能力。
Objective： To explore the effect of epigallocatechin-3-gallate （EGCG） on the proliferation and radioiodine accumulation in papillary thyroid cancer（PTC） cells. Methods： After PTC cells W3 were treated by using different concentrations of EGCG for 48 h, the proliferation of PTC cells was detected by 3- 4,5-dimethylthiazol-2-yl-2,5-diphenyl-tetrazolium bromide （MTF） assay ; the expression of sodium iodide symporter（NIS） in PTC cells was detected by flowcytometry and immunofluorescence methods; the ability of PTC cells to iodide-uptake was measured by iodide-uptake assay. Results： The proliferation of PTC cells was decreased, and the expression of NIS in VI＇C cells was increased, in a concentration dependent manner. And the ability of iodide-uptake in PTC cells was increased significantly in some concentration gradient. Conclusion： EGCG inhibited the growth of PTC cells and enhanced the ability of iodide-uptake through increased expression of NIS.
出处
《江苏大学学报（医学版）》
CAS
2017年第1期10-13,共4页
Journal of Jiangsu University：Medicine Edition
基金
国家自然科学基金资助项目(81370889)
