摘要
目的探讨中国西北汉族人群中胰岛素样生长因子2结合蛋白2（IGF2BP2）基因多态性与代谢综合征（MS）及其表型的关系。方法采用病例-对照研究方法，随机数字表法选取2007至2008年陕西省糖尿病流行病调查数据库350例MS患者和304例非MS对照人群，应用基质辅助激光解吸电离飞行时间质谱技术对病例组及对照组IGF2BP2基因多态位点rs4402960、rs1470579进行基因分型。采用卡方检验比较MS组和正常对照组rs4402960、rs1470579基因型与等位基因分布频率的差异；采用Logistic回归分析rs4402960、rs1470579与MS参数个数的关系，采用线性回归分析rs4402960、rs1470579与MS组分的关系。结果基因频率分析显示，rs4402960位点的等位基因T频率在MS组及对照组分别为28.6%和27.3%，两组间差异无统计学意义（χ^2=0.268，P=0.605），rs1470579位点的等位基因C频率在MS组及对照组分别为30.1%和29.3%，两组间差异亦无统计学意义（χ^2=0.117，P=0.732），研究未发现IGF2BP2基因多态性与MS有相关性。线性回归分析显示，经校正年龄、性别及体质指数、吸烟、饮酒及锻炼后，IGF2BP2基因rs4402960在隐性模型（t=2.068，P=0.040）及加性模型（t=2.144，P=0.034）下可见与收缩压的相关趋势。结论中国西北汉族人群中IGF2BP2基因多态位点rs4402960、rs1470579与MS无明显关联，但IGF2BP2基因rs4402960多态性位点的T等位基因可能与收缩压相关。
ObjectiveTo investigate the association of insulin-like growth factor 2 mRNA-binding protein 2 （IGF2BP2） gene polymorphisms with metabolic syndrome （MS） and its related phenotypes in Han population in northwest China.MethodsA case-control study was performed, which included 350 subjects with MS （MS group） and 304 subjects without MS （control group） selected with random number table from the epidemiological survey database of diabetes in Shannxi province form 2007 to 2008. Matrix assisted laser desorption/ionization time-of-flight mass spectrometry （MALDI-TOF MS） was used as the genotyping method. rs4402960 and rs1470579 genotypes and allele frequencies between MS group and control group were analyzed. Logistic regression was used to analyze the associations of different genotypes of rs4402960 and rs1470579 with parameters of MS. Linear regression was used to analyze the relationship between IGF2BP2 gene rs4402960 and rs1470579 polymorphisms and parameters of MS.ResultsNo single nucleotide polymorphism was found to be associated with MS. The T allele frequencies of rs4402960 in MS group and control group were 28.6% and 27.3%, respectively （χ^2=0.268, P=0.605）. The C allele frequencies of rs1470579 in MS group and control group were 30.1% and 29.3%, respectively（χ^2=0.117, P=0.732）. With the linear regression analysis adjusted for age, gender, body mass index, smoking and physical exercise, a tendency toward association between rs4402960 and systolic pressure was observed under recessive model （t=2.068, P=0.04） and additive model（t=2.144, P=0.034）.ConclusionsIGF2BP2 rs4402960 and rs1470579 polymorphisms are not associated with MS in Han population in northwest China. However, IGF2BP2 may be related to increased systolic blood pressure.
出处
《中华糖尿病杂志》
CAS
CSCD
2017年第3期169-173,共5页
CHINESE JOURNAL OF DIABETES MELLITUS
基金
国家自然科学基金青年科学基金（81300696）
陕西省科技统筹创新工程计划项目课题（2013KTZB03-02-01）
西京医院学科助推计划转化医学课题（XJZT13204）
关键词
代谢综合征
胰岛素样生长因子2结合蛋白2基因
多态性
单核苷酸
胰岛素抵抗
收缩压
Metabolic syndrome
Insulin-like growth factor 2 mRNA-binding protein 2 gene
Polymorphism, single nucleotide
Insulin resistance
Systolic blood pressure
