摘要
目的探讨不同程度间歇低氧(IH)对MLg小鼠肺成纤维细胞活化和胞外基质分泌的影响。方法取对数生长期的MLg成纤维细胞,IH分别处理1 h、4 h、8 h(IH1组、IH4组、IH8组),IH频率为5%O_2100 s,21%O_2120 s,并设置常氧对照组(21%O_2处理8 h,N组)。实验结束后收集细胞,采用q RT-PCR和Western blot检测各组α肌动蛋白(α-SMA)、Ⅰ型胶质蛋白(COL1)m RNA和蛋白表达水平。结果 IH1组、IH4组、IH8组α-SMA、COL1 m RNA及蛋白的表达水平明显高于N组(P<0.05),且随着IH时间的延长,α-SMA、COL1表达水平逐渐升高。结论 IH可以促进小鼠肺成纤维细胞活化和胞外基质分泌,其作用机制可能与氧化应激有关。
Objective To explore the effects of different degrees of intermittent hypoxia（IH） on the activation and thesecretion of extracellular matrix in MLg lung fibroblast cell line. Methods MLg lung fibroblast cells in logarithmic growthphase were exposed for 5% O2 for 100 seconds and 21% O2 for 120 seconds in 1 h, 4 h and 8 h groups（IH1, IH4 and IH8）and normoxia group（21% O2 for 8 h, N group）. The cells in each group were collected at the end of experiment. Real-timePCR was used to measure the m RNA expression levels of α-SMA and type Ⅰ collagen（COL1） A1, and Western blot assaywas used to detect the protein expression levels of α-SMA and COL1. Results The m RNA and protein expression levels ofα- SMA and COL1 were significantly increased in IH1, IH4 and IH8 groups than those in N group（all P 〈 0.05）.Furthermore, expression levels of α- SMA and COL1 showed a time- dependent increase with IH exposure time.Conclusion The intermittent hypoxia can promote the cell activation and the extracellular matrix secretion of mouse lungfibroblast cells, which may be related with the oxidative stress.
出处
《天津医药》
CAS
2017年第1期9-12,共4页
Tianjin Medical Journal
基金
国家自然科学基金资助项目(81500070
81670084)
天津医科大学总医院青年孵育基金(ZYYFY2014011)
