摘要
目的探讨富硒酵母对大气细颗粒物(PM_(2.5))致大鼠肺损伤的干预作用。方法 56只健康成年SD大鼠随机平均分为7组:生理盐水对照组(气管滴注生理盐水);PM_(2.5)染毒组(气管滴注PM_(2.5)混悬液,40 mg/kg,隔天一次,共3次);溶剂对照组(1%羧甲基纤维素灌胃);低、中、高剂量干预组(气管滴注PM_(2.5)混悬液,末次滴注24h后,分别灌胃给予富硒酵母8.75、17.5和35 mg/kg,每天1次,共7d);干预对照组(35 mg/kg富硒酵母灌胃)。末次灌胃24 h后,收集肺泡灌洗液(BALF),测定BALF中肿瘤坏死因子-α(TNF-α)、白细胞介素-1β(IL-1β)、丙二醛(MDA)、总蛋白(TP)含量及总超氧化物歧化酶(T-SOD)、谷胱甘肽过氧化物酶(GSH-Px)、乳酸脱氢酶(LDH)及碱性磷酸酶(AKP)活性,并计数细胞。未灌洗肺叶制作病理切片。结果与对照组相比,PM_(2.5)染毒组及低、中、高剂量干预组BALF中中性粒细胞百分比、TNF-α、IL-1β、TP及MDA含量,LDH及AKP活性增高,GSH-Px及T-SOD活性下降(P<0.05)。与PM_(2.5)染毒组相比,干预组BALF中中性粒细胞百分比、TNF-α、IL-1β、TP及MDA含量,LDH及AKP活性降低,GSH-Px及T-SOD活性升高(P"0.05),随干预剂量增加,中性粒细胞百分比、TNF-α、IL-1β、TP及MDA含量,LDH及AKP活性降低,GSH-Px、T-SOD活性升高幅度增加。结论富硒酵母可适当减轻PM_(2.5)所致大鼠肺组织炎性损伤及氧化应激损伤。
Objective To evaluate if selenium yeast could inhibit the rat lung injury induced by ambient fine particulate matter（ PM2.5 ）. Methods Fifty-six male SpragueDawley rats were randomly allocated in seven groups（ n = 8 each）. Saline control group,the rats were exposed to 0. 9% saline by instillation. PM2.5 exposure group,rats were exposed to PM2.5 by intra-tracheal instillation every other day for three times with the accumulated dose of 40 mg/kg. Selenium yeast treatment groups,three groups of rat were exposed to PM2.5 . Then the rats were given low,middle and high dose of selenium yeast,and the doses were 8. 75,17. 5 and 35 mg/kg,respectively. High dose selenium yeast control group,rats were given high dose of selenium yeast only. Solvent control group,therats were given 1% carboxymethyl cellulose. Saline and PM2.5 were given in the first week. In the second week,selenium yeast and solvent were given by gavage. The rats were sacrificed 24 hours after the last gavage. The bronchoalveolar lavage fluid（ BALF）was collected to count the neutrophils numbers and analyze the markers related to inflammation,oxidative stress and cell damage. The lung lobe that was not been lavaged was processed for light microscopic examination. Results The proportions of neutrophils in BALF and the pathologic scores of lung in PM2.5 - exposed groups were significantly higher than control（ P〈0. 05）. Selenium yeast treatment caused decrease in tumor necrosis factor-α（ TNF-α）,interleukin-1β（ IL-1β）,lactate dehydrogenase（ LDH）,total protein（ TP）,alkaline phosphatase（ AKP） and malondialdehyde（ MDA） compared with the only PM2.5 exposure group. Meanwhile,the dose-dependent increase in totalsuperoxide dismutase（ T-SOD） and glutathione peroxidase（ GSH-Px） activities were observed. There were no significant differences among the groups of saline control group,high dose selenium yeast control group and solvent control group. Conclusion Selenium yeast treatment may protect against acute injury indu
出处
《卫生研究》
CAS
CSCD
北大核心
2017年第2期219-224,231,共7页
Journal of Hygiene Research
基金
国家自然科学基金(No.91543119)
关键词
细颗粒物
肺损伤
富硒酵母
干预
fine particulate matter
lung injury
selenium yeast
intervention effects
