摘要
In recent decades,a cardiomyocyte membrane scaffolding protein bridging integrator 1(BIN1) has emerged as a critical multifunctional regulator of transverse-tubule(t-tubule) function and calcium signaling in cardiomyocytes.Encoded by a single gene with 20 exons that are alternatively spliced,more than ten BIN1 protein isoforms are expressed with tissue and disease specificity.The recently discovered cardiac alternatively spliced isoform BIN1(cBIN1 or BIN1 +13 + 17)plays a crucial role in organizing membrane microfolds within cardiac t-tubules.These cBIN1-induced microfolds form functional dyad microdomains by trafficking L-type calcium channels(LTCC) to t-tubule membrane and recruiting ryanodine receptors(RyR) to junctional sarcoplasmic reticulum membrane.When cBIN1 is transcriptionally reduced as occurs in heart failure,cBIN1-microfolds are disrupted and fail to form LTCC and RyR couplons.As a result,impaired dyad formation limits excitation-contraction coupling thus cardiac contractility,and accumulation of orphaned leaky RyRs outside of dyads increases ventricular arrhythmias.Reduced myocardial BIN1 in heart failure is also detectable at the blood level,and plasma BIN1 level predicts heart failure progression and future arrhythmias in cardiomyopathy patients.Here we will review the recent progress in BIN1-related cardiomyocyte biology studies and discuss the diagnostic and predictive values of cBIN1 in future clinical use.
In recent decades,a cardiomyocyte membrane scaffolding protein bridging integrator 1（BIN1） has emerged as a critical multifunctional regulator of transverse-tubule（t-tubule） function and calcium signaling in cardiomyocytes.Encoded by a single gene with 20 exons that are alternatively spliced,more than ten BIN1 protein isoforms are expressed with tissue and disease specificity.The recently discovered cardiac alternatively spliced isoform BIN1（cBIN1 or BIN1 ＋13 ＋ 17）plays a crucial role in organizing membrane microfolds within cardiac t-tubules.These cBIN1-induced microfolds form functional dyad microdomains by trafficking L-type calcium channels（LTCC） to t-tubule membrane and recruiting ryanodine receptors（RyR） to junctional sarcoplasmic reticulum membrane.When cBIN1 is transcriptionally reduced as occurs in heart failure,cBIN1-microfolds are disrupted and fail to form LTCC and RyR couplons.As a result,impaired dyad formation limits excitation-contraction coupling thus cardiac contractility,and accumulation of orphaned leaky RyRs outside of dyads increases ventricular arrhythmias.Reduced myocardial BIN1 in heart failure is also detectable at the blood level,and plasma BIN1 level predicts heart failure progression and future arrhythmias in cardiomyopathy patients.Here we will review the recent progress in BIN1-related cardiomyocyte biology studies and discuss the diagnostic and predictive values of cBIN1 in future clinical use.
基金
supported by the United States National Institute of Health/National Heart,Lung,and Blood Institute (NIH/NHLBI,Hong R01 HL133286)
American Heart Association (AHA)(IRG27780031,BGIA27770151)
