摘要
目的乙肝病毒X蛋白结合蛋白（hepatitis BX-interacting protein,HBXIP）在多种肿瘤中存在过度表达,且其过表达与肿瘤的恶性进展相关。本研究旨在探讨HBXIP过表达与膀胱尿路上皮癌临床病理特征和预后的关系。方法收集延边大学医院2010-01-01-2014-12-31有完整临床病理资料,且病理检查确诊为膀胱尿路上皮癌的100例癌组织作为研究组,10例同期癌旁组织作为对照组;另取10例配对的新鲜癌组织及癌旁组织作为研究组及对照组。采用免疫组化SP方法、蛋白质印迹法和逆转录-聚合酶链反应（reverse transcription-polymerase chain reaction,RT-PCR）方法检测HBXIP的表达,分析其与肿瘤病理分级、分期等临床病理特征的关系,同时研究HBXIP表达水平与膀胱尿路上皮癌患者预后的关系。结果免疫组化结果显示,HBXIP在膀胱尿路上皮癌中的高表达率为86.0%（86/100）,明显高于癌旁正常膀胱组织中的20.0%（2/10）,两组比较差异有统计学意义,P=0.036;同时,肌层浸润组中HBXIP高表达率为79.3（23/29）,明显高于非肌层浸润组的50.7%（36/71）,两组比较差异有统计学意义,P=0.046。蛋白质印迹法检测结果显示,膀胱尿路上皮癌中HBXIP的相对表达量为0.712±0.135,明显高于癌旁正常组织（0.326±0.075）,两组比较差异有统计学意义,P=0.047。RT-PCR实验结果显示,膀胱尿路上皮癌中HBXIP mRNA的表达量为5.46±0.34,明显高于癌旁正常组织（1.01±0.20）,两组比较差异有统计学意义,P=0.041;HBXIP高表达率与肿瘤浸润深度（P=0.048）及淋巴结转移（P=0.036）呈正相关;HBXIP高表达患者术后复发率为42.5%（20/47）,而HBXIP低表达患者术后复发率仅为15.1%（8/53）,两组比较差异有统计学意义,P=0.003。影响患者生存率的单因素分析显示,HBXIP高表达组膀胱尿路上皮癌患者术后生存率明显低于HBXIP低表达组,两组比较差异有统计学意义,P=0.014;肌层浸润组（T2～T3
OBJECTIVE The over expression of hepatitis BX-interacting protein（HBXIP）is detected in a variety of tumors and it is closely related with the development,invasion and metastasis of tumors.The purpose of this article was to explore the correlation between the over expression of HBXIP and the tumor size,lymph node metastasis,clinical stage,pathological grades and prognosis of bladder urothelial carcinoma（BUC）.METHODS The cases came from Yanbian University Hospital with complete clinical and pathological information from January 1,2010 to December 31,2014.Totally 100 cases of BUC tissue were as a study group,10 cases of adjacent normal tissue were as a control group in the same period.Another 10 cases of frozen BUC tissue and matched paracarcinoma tissue were collected as study group and control group.Immunohistochemical SP method,Western blot and reverse transcription polymerase chain reaction（RT-PCR）were used to detect the expression of HBXIP.The correlations between the HBXIP expression and the clinical pathological features and prognosis of the patients were analyzed.RESULTS The immunochemistry results showed that the over expression of HBXIP was 86.0%（86/100）in BUC and 20.0%（2/10）in adjacent normal tissues respectively with a significant difference between two groups（P=0.036）.The over expression of HBXIP was 79.3%（23/29）in muscle invasive group and 50.7%（36/71）in non-muscle invasive group,the difference was significant（P=0.046）.Western blot showed that the relative quantitative expression of HBXIP was 0.712±0.135 in BUC and 0.326±0.075 in paracarcinoma tissue,the difference was significant（P=0.047）.RT-PCR showed that the quantitative expression of HBXIP mRNA was 5.46±0.34 in BUC and 1.01±0.20 in paracarcinoma tissue,the difference was significant（P=0.041）.The over expression of HBXIP was positive correlation with the depth of tumor invasion（P=0.048）and lymph node metastasis（P=0.036）.We also found that the postoperative recurrence rate was 42.5%（20/47）i
作者
李晓刚
刘双萍
孙抒
LI Xiao gang LIU Shuang-ping SUN Shu(Department of Urology, Hospital of Yanbian University ,Yanji 133000, P. R. China Department of Pathology ,Yanbian University, Yanji 133000 ,P. R. China)
出处
《中华肿瘤防治杂志》
CAS
北大核心
2016年第24期1607-1612,共6页
Chinese Journal of Cancer Prevention and Treatment
关键词
膀胱尿路上皮癌
乙肝病毒X蛋白结合蛋白
侵袭和转移
预后
bladder urothelial carcinoma
hepatitis BX-interacting protein（HBXIP）
invision and metastasis
prognosis
