摘要
目的：探讨乙肝病毒X蛋白结合蛋白HBXIP和细胞凋亡调节因子GRIM-19在肝细胞癌组织中的表达及意义。方法收集42例肝细胞癌组织和28例正常肝脏组织，采用免疫组织化学法检测两种组织中HBXIP和GRIM-19的表达。结果在肝细胞癌组织和正常肝脏组织中，HBXIP阳性表达率分别为80.95%（34/42）和42.86%（12/28）；而GRIM-19阳性表达率分别为40.48%（17/42）和75.00%（21/28），差异有统计学意义（P＜0.01）。肝细胞癌组织中HBXIP的阳性表达率在低、未分化和Ⅲ～Ⅳ期的表达水平高于高、中分化和Ⅰ～Ⅱ期组织；而GRIM-19的阳性表达率与之相反，且无门脉癌栓组织中GRIM-19的阳性表达率高于有门脉癌栓组织。HBXIP与GRIM-19表达呈负相关（rs=-0.400，P＜0.01）。结论 HBXIP和GRIM-19的异常表达可能与肝细胞癌的发生及浸润转移有密切关系。
Objective To investigate the expressions of HBXIP and GRIM-19 in hepatocellular carcinoma tissues and their clinic significance. Methods Hepatocellular carcinoma tissue （n=42） and normal liver tissue （n=28） were collected from Tianjin First Central Hospital,immunohistochemistry was used to detect the expressions of HBXIP and GRIM-19 in these two groups. Results Rate of cells with positive expressions of HBXIP in hepatocellular carcinoma and normal liver tissues were 80.95%（34/42）and 42.86%（12/28）respectively;Rate of cells with positive expression of GRIM-19 in hepato？cellular carcinoma tissues and normal liver tissues was 40.48%（17/42）and 75.00%（21/28）respectively, and the difference between these two groups was statistically significant（P〈0.05）;The expression of HBXIP was higher but the expression of GRIM-19 was lower in poor differentiated and stageⅢ-IV cells than those in well and moderate differentiated cells and in stage I-II, cells. What′s more, the expression of GRIM-19 is higher in tissue without portal thrombosis than that in tissue with portal thrombosis. The expression of HBXIP was negatively correlated with GRIM-19 expression（rS=-0.400,P〈0.01）. Conclusion The abnormal expressions of HBXIP and GRIM-19 may play important roles in the process of development and metastasis of hepatocellular carcinoma.
出处
《天津医药》
CAS
2015年第12期1364-1367,共4页
Tianjin Medical Journal
基金
天津市科技计划项目(10ZCGYSF00500)
