摘要
目的探讨甜菜碱补充对高S-腺苷同型半胱氨酸(SAH)诱导的动脉粥样硬化(AS)的影响。方法采用apo E基因敲除(apoE^(-/-))小鼠制备AS模型,饮食添加高蛋氨酸和SAH水解酶抑制剂建立高SAH模型,在SAH模型基础上饮食添加不同剂量甜菜碱干预,观察小鼠血脂水平、血浆SAH、S-腺苷蛋氨酸(SAM)、同型半胱氨酸(Hcy)浓度以及SAM/SAH比值变化,采用油红O染色观察apoE^(-/-)小鼠主动脉窦AS斑块情况。结果与对照组比较,高SAH模型组小鼠血浆SAH水平[(78.61±4.62)nmol/L]升高,SAM水平[(40.17±3.02)nmol/L]及SAM/SAH比值(0.65±0.11)降低,Hcy水平[(6.97±0.51)μmol/L]无明显变化,AS斑块绝对面积[(52.65±8.40)mm^2]和相对面积(25.47±5.23)%均增加;与高SAH模型组比较,高剂量甜菜碱组小鼠血浆SAH水平[(30.18±3.85)nmol/L]、Hcy水平[(4.91±0.30)μmol/L]降低,SAM水平[(78.14±3.99)nmol/L]及SAM/SAH比值(2.78±0.34)升高,AS斑块绝对和相对面积[(28.75±7.24)mm^2、(13.89±3.96)%]下降;呈剂量-效应关系。结论甜菜碱干预可降低apoE^(-/-)小鼠血浆高SAH水平,对高SAH诱导的动脉粥样硬化具有缓解作用。
Objective To explore the effect of betaine supplementation against high S-adenoslhomocysteine （SAH）-induced atherosclerosis （AS）.Methods Dietary supplementation of high methionine and SAH hydrolase inhibitor was used to establish a model of high SAH-induced AS in apoE-deficient （apoE-/-） mice.The mice with high plasma SAH level were treated with betaine at three different doses.After 8 and 16 weeks＇ betaine treatment,blood samples of the mice were collected to measure serum lipids levels,plasma SAH,S-adenoslmethionine （SAM）,and homocysteine （Hcy） concentrations.The hearts of apoE-/- mice were examined to assess the AS plaque area by staining with oil red O.Results Compared with the control group,the mice of high SAH group showed increased plasma SAH concentration（78.61±4.62 nmol/L）,decreased SAM concentration （40.17±3.02 nmol/L）,and decreased SAM/SAH ratio （0.65±0.11）;there was no significant change in Hcy concentration （6.97±0.51μmol/L）,while there were increases in absolute plaque area （52.65±8.40 mm2） and relative AS plaque area （25.47±5.23%） in the mice of high SAH group.Compared with high SAH group,the mice with high-dose betaine supplementation had significantly decreased plasma SAH （30.18±3.85 nmol/L） and Hcy （4.91±0.30μmol/L）,increased SAM （78.14±3.99 nmol/L） and SAM/SAH ratio （2.78±0.34）,and reduced high SAH-induced absolute AS plaque area （28.75±7.24）mm2 and relative AS plaque area （13.89±3.96%）,in a dose-response dependent manner.Conclusion Betaine supplementation could signifi-cantly decrease high plasma SAH level and reduce high SAH-induced AS plaque area in apoE-/- mice.
作者
肖云军
黄海雄
吴小敏
张锦周
黄薇
黄海燕
凌文华
XIAO Yun-jun HUANG Hai-xiong WU Xiao-min et al(Department of Nutrition and Food Hygiene, Shenzhen Mu- nicipal Center for Disease Control and Prevention, Shenzhen, Guangdong Province 518055, China)
出处
《中国公共卫生》
CAS
CSCD
北大核心
2017年第3期478-481,共4页
Chinese Journal of Public Health
基金
国家自然科学基金(81402672)
