摘要
为探究纤维素降解菌黑葡萄穗霉的环境适应机制,分别将该属菌株S05培养于不同pH、不同碳源培养基中,检测其生长及胞外糖苷水解酶表达情况,并利用糖苷水解酶活性电泳展示各主要酶组分的多样性及其催化性质.结果显示,黑葡萄穗霉S05生长pH范围较宽,在pH 4.5-9.5范围内均能生长,且更适于中性偏碱性环境;胞外主要糖苷水解酶组分最适pH多为pH 6.5-8.5,pH稳定性较强;该菌株具有精细的表达调控体系,能及时根据环境条件的变化调整酶组分的组成.综上表明黑葡萄穗霉S05的高环境适应性与其胞外糖苷水解酶组分的性质密切相关,对其酶组分的深入研究可为寻找工业所需中性及偏碱性纤维素酶的来源提供可能.
This work aimed to find out the reasons for the wide distribution and strong environment adaptiveness of the cellulose-degrading strains of Stachybotrys chartarum. We analyzed the growth state and expression of glycoside hydrolases by cultivating strain S05 of S. chartarum in cellulose media with different pH values or supplemented with different substrates as the sole carbon source. The diversity and the intrinsic properties of glycoside hydrolases were demonstrated through NativePAGE. The S. chartarum strain S05 exhibited adaptiveness in a wide pH range of pH 4.5-9.5, more adaptive to neutral or alkali environments. It secreted a wide range of glycoside hydrolases, in which the main components have neutral optimal pH values from pH 6.5 to pH 8.5, and wide pH stability ranges. This was partly because strains of this species had a fine-tuned expression system to accurately adjust the composition of enzymes according to the changes of environment. This study demonstrated the strong environment adaptiveness character of the cellulose-degrading species of S. chartarum and showed its close relation to the properties of extracellular glycoside hydrolases. Further analysis of the enzymes will provide great potentials to meet the industrial needs for neutral or alkaline cellulases.
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2017年第1期117-122,共6页
Chinese Journal of Applied and Environmental Biology
基金
国家自然科学基金项目(6622316033)
青岛农业大学高层次人才启动基金项目(631342)资助~~
关键词
黑葡萄穗霉
糖苷水解酶
活性电泳
中性纤维素酶
Stachybotrys chartarum
glycoside hydrolases
Native-PAGE
neutral cellulases
