摘要
试验以45°C老化48d的超干(含水量4%)燕麦(Avena sativa)种子为材料,通过不同浓度PEG-6000(0,-0.3,-0.6,-0.9和-1.2 Mpa)引发0(CK),3和6h后,分析其超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)活性及丙二醛(MDA)含量的变化,以探讨PEG引发对老化的超干燕麦种子抗氧化性能的影响。结果表明:浓度为0和-0.3 Mpa的PEG引发会降低SOD,CAT和POD的活性,导致MDA含量增加,从而降低老化燕麦种子发芽率;而高浓度(-0.9和-1.2 Mpa)的PEG引发则相反。说明适当浓度的PEG引发主要通过提高超干燕麦种子的抗氧化能力来修复其老化损伤,并避免其吸胀损伤。
This study was designed ：to analyze the activities of superoxide dismutase （SOD）, catalase （CAT）, peroxidase （POD）, and malondialdehyde （MDA） content in ultra-dried （4% moisture content） oat （Arena sativa） seeds. The seeds were deteriorated at 45℃ for 48 d, and then primed with different concentrations of PEG-6000 （0, -0.3, -0.6, -0.9 and -1.2 Mpa） for 0 （CK）, 3 and 6 h. This study was to explore the effect of PEG priming on physiological responses of aged ultra-dry oat seeds. The re- sults showed that the activities of SOD, CAT and POD decreased in aged oat seeds primed with PEG at the concentration of 0 and -0.3 Mpa, leading to an increase in MDA content and decrease in germination per- centage. However, the opposite trend was observed after priming with high concentration of PEG （-0.9 and -1.2 Mpa）. This indicated that PEG priming repaired the damage of ageing and prevented the imbihi- tion damage in ultra-dried oat seeds by improving antioxidant capacity.
作者
夏方山
毛培胜
王明亚
闫慧芳
侯龙鱼
陈玲玲
朱艳乔
汪辉
XIA Fang-shan MAO Pei-sheng WANG Ming-ya YAN Hui-fang HOU Long-yu CHEN Ling-ling ZHU Yan-qiao WANG Hui(College of Animal Science and Veterinary Medicine, Shanxi Agricultural University, Taigu, Shanxi Province 030801, China College of Animal Science and Technology, China Agricultural University, Beijing Key Laboratory of Grassland Science, Beijing 100193, China)
出处
《草地学报》
CAS
CSCD
北大核心
2016年第5期933-938,共6页
Acta Agrestia Sinica
基金
博士点基金项目(20110008110003)
国家"十二五"科技支撑课题"优质多抗牧草新品种选育与良种繁育关键技术研究与示范(2011BAD17B01-02)"资助
关键词
燕麦
引发
聚乙二醇
种子老化
超干
Oat
Priming
Polyethylene glycol
Seed ageing
Ultra-dry
