摘要
目的探讨应用基质辅助激光解吸电离飞行时间技术质谱（MALDI—TOFMS）在临床微生物快速检测中的应用价值。方法收集新疆昌吉回族自治州人民医院近两年的分离菌株525株，分别分离自痰、尿、血液、分泌物、脓液等临床标本，其中革兰阴性（G-）细菌225株，革兰阳性（G-）细菌225株，酵母菌75株。将MALDI—TOFMS与VITEK2 Compact全自动微生物分析系统（VITEK2 Compact）鉴定结果进行比较。结果G-细菌鉴定符合率为96．4％（217／225），G’细菌鉴定符合率为94．2％（212/225），酵母菌鉴定符合率为94．7％（71／75）。结论MALDI-TOFMS技术快速、简便、准确且价格低廉，在快速鉴定临床常见病原菌方面，尤其是在危重症患者的快速检测方面具有广阔的应用前景，为临床救治争取了宝贵的时间。然而目前该技术的应用仍有一定的局限性，其检测的准确性受被测菌纯度、实验操作等影响，且对某些菌种的鉴定准确性不高，对病毒检测的过程较复杂，因此MALDI—TOFMS技术仍需进一步的探索研究。
Objective To explore the application value of the matrix assisted laser desorption ionization time of flight mass spectrometer （MALDI-TOF MS） in rapid detection of clinical microbiology . Methods 525 isolation strains were collected in People＇s Hospital of Changji Hui Autonomous Prefecture in the past two years, that were isolated from sputum, urine, blood, secretions and pus in clinical specimens respectively, Among them , 225 strains of gram negative bacteria, 225 strains of gram positive bacteria, 75 strains of yeast. The MALDI-TOF MS identification results and VITEK2 Compact identification results were compared . Results The compliance rate of MALDI-TOF MS and VITEK2 Compact, gram negative bacteria was 96.4% （217/225）, gram positive bacteria was 94.2% （212/225） , yeast was 94.7% （71/75） . Conclusions MALDI-TOF MS technology is rapid, simple, accurate and the price is low, it will have broad prospect of application in rapid identification of clinical common pathogenic bacteria especially in rapid detection of critically ill patients, and took precious time for clinical treatment. However, the application of this technology still has some limitations, it＇s accuracy was affected by the purity of tested bacteria and the ability in experimental operation, and has a low accuracy for some species, the process of detection is more complex for virus [1], therefore MALDI-TOF MS technology still need a further study.
作者
阎萍
王萍
王旭晖
YAN Ping WANG Ping WANG Xu-hui(Department of Clinical Laboratory, People＇s Hospital of Changji Hui Autonomous Prefecture, Changji Hui Autonomous Prefecture 831100, Xinjiang, China)
出处
《实用检验医师杂志》
2016年第4期193-195,共3页
Chinese Journal of Clinical Pathologist
基金
新疆昌吉回族自治州科技开发计划项目(2016S08-01)
关键词
微生物
基质辅助激光解吸电离飞行时间技术质谱
快速检测
Microbiology. matrix assisted laser desorpfion ionization time of flight mass spectrometer. Rapid detection
