摘要
目的探讨心理应激后大鼠血清蛋白表达谱的变化特点。方法 24只雄性Wistar大鼠随机分为心理应激组、对照组、应激源组。采用大鼠旁观电击模型,对应激源组大鼠进行间歇性足底电击刺激;心理应激组大鼠不受电击,通过视觉、听觉、嗅觉系统接收来自应激源组的信息,引起心理应激;对照组大鼠单独喂养,不接受任何处理。实验结束后取心理应激组和对照组大鼠血清,采用表面加强激光解吸电离飞行时间质谱(SELDI-TOF-MS)芯片技术检测蛋白表达谱的变化并进行统计学分析。结果两组血清中质荷比(M/Z)为8870.18、6860.85、8625.13、11413.91Da的4种蛋白质相对含量有显著差异(P<0.05),心理应激组中这4种蛋白质表达均下调。结论心理应激可使血清蛋白质表达谱发生变化。
Objective To explore the changes in serum protein profiles in rats after psychological stress. Methods Twenty-four male Wistar rats were randomly divided into psychological stress group,control group and stressor group. Rats in stressor group received intermittent foot-shock. Instead of foot-shock,the rats in psychological stress group received the stimulating information from stressor group by watching,listening and smelling. Rats in control group were kept in individual cages and received no stimulation. Experiments were performed once every morning for two weeks. Serum samples of rats in both psychological stress group and control group were obtained and the changes in protein profiles were determined and statistically analyzed by Surface Enhanced Laser Desorption Ionization-Time of Flight-Mass Spectrometry （SELDI-TOF-MS） after the experiments. Results Variance analysis showed that the relative contents of proteins with M/Z values of 8870.18,6860.85,8625.13 and 11 413.91Da decreased significantly in rats of psychological stress group as compared with control group （P0.05）. The expressions of the 4 proteins were down-regulated in the rats of psychological stress group. Conclusion Psychological stress may affect the serum protein profiles of serum of rats.
出处
《解放军医学杂志》
CAS
CSCD
北大核心
2010年第8期958-960,共3页
Medical Journal of Chinese People's Liberation Army
基金
济南军区"十一五"科技研究课题(J115N025)
关键词
应激
心理学
蛋白质组学
光谱法
质量
基质辅助激光解吸电离
stress
psychological
proteomics
spectrometry
mass
matrix-assisted laser desorption-ionization
