摘要
目的探讨心肌缺血-致命性心动过缓大鼠血清代谢特征。方法制作心肌缺血-心动过缓-死亡(myocardial ischemia-bradycardia-sudden cardiac death,MI-B-SCD)大鼠模型,以假手术组为对照组,应用气相色谱质谱联用仪检测死后血清代谢谱,结合代谢组学策略分析血清代谢特征。结果 MI-B-SCD大鼠血清代谢轮廓与对照差异显著。与假手术组相比,MI-B-SCD大鼠血清赖氨酸、鸟氨酸、嘌呤、丝氨酸、丙氨酸、尿素、乳酸含量明显升高;琥珀酸、棕榈酸、酮己二酸、甘油醛、己烯二酸、辛二酸含量明显下降,其中多种差异代谢物之间存在相关性。结论 MI-B-SCD大鼠血清代谢显著改变,赖氨酸与嘌呤有较高的诊断MI-B-SCD价值,可望为心源性猝死的法医鉴定及临床防治提供参考。
Objective To explore the metabolic characteristics of lethal bradycardia induced by myocardial ischemia in rat＇s serum. Methods A rat myocardial ischemia-bradycardia-sudden cardiac death（MI-BSCD） model was established, which was compared with the sham-operation group. The metabolic profile of postmortem serum was analyzed by gas chromatography-mass spectrometry（GC-MS）, coupled with the analysis of serum metabolic characteristics using metabolomics strategies. Results The serum metabolic profiles were significantly different between the MI-B-SCD rats and the control rats. Compared to the control rats, the MI-B-SCD rats had significantly higher levels of lysine, ornithine, purine, serine, alanine,urea and lactic acid; and significantly lower levels of succinate, hexadecanoic acid, 2-ketoadipic acid,glyceraldehyde, hexendioic acid and octanedioic acid in the serum. There were some correlations among different metabolites. Conclusion There is obvious metabolic alterations in the serum of MI-B-SCD rat.Both lysine and purine have a high value in diagnosing MI-B-SCD. The results are expected to provide references for forensic and clinical applications of prevention and control of sudden cardiac death.
出处
《法医学杂志》
CAS
CSCD
2017年第1期11-16,20,共7页
Journal of Forensic Medicine
基金
广东省自然科学基金资助项目(2015A030313437)
广东省科技创新资助项目(2013KJCX0076)
关键词
法医病理学
心肌缺血
心动过缓
血清
代谢
forensic pathology
myocardial ischemia
bradycardia
serum
metabolic
