摘要
目的通过检测不同期别矽肺患者和接尘工人痰液单核细胞炎性蛋白-1(MCP-1)、巨噬细胞趋化蛋白-1α(MIP-1α)含量变化及与肺通气功能相关性,探讨两种炎性趋化因子与矽肺发病的关系。方法将不同期别矽肺患者及有无接尘工人分为5组;用痰液诱导(sputum-induction,SI)的方法获取标本;以酶联免疫吸附方法测定痰中MCP-1、MIP-1α的含量。结果 MIP-1α及MCP-1组间差异均存在统计学意义(F=3.111,P=0.016;F=6.865,P=0.000)。MIP-1α与肺功能指标FEV_(1.0)、FEV_(1.0)/VCmax、MEF_(50)、MEF_(25)均呈明显正相关关系(P<0.05)。结论 MCP-1、MIP-1α可作为反映矽肺病程进展的敏感性生物指标,MIP-1α的表达水平更具有临床应用价值。
Objective Through detecting the contents of MCP-1,MIP-1α in sputum of silicosis patients,silica-exposed workers and non silica-exposed workers,analyzing their correlations with pulmonary ventilation function,and to explore the relationship of these two inflammatory factors with the occurrence and development of silicosis. Methods The silicosis patients,silica-exposed and non silica exposed workers were divided into five groups,repectively,sputum-induction（SI） method was used to obtain sputum samples and enzyme linked immunosorbent assay to determine the contents of MCP-1,MIP-1α in sputum. Results There were significant differences between groups both the contents of MIP-1 and MIP-1α（F = 3. 111,P = 0. 016 and F = 6. 865,P = 0. 000,respectively）,and MIP-1α and pulmonary function indexes such as FEV（1.0）,FEV（1.0）/VCmax,MEF（50）,MEF（25） all showed significant positive correlation（P〈0. 05）. Conclusion The results suggested that the MIP-1 and MCP-1 could be used as a sensitive index to reflect the course of the silicosis,especially MIP-1α which may have more clinical significance.
出处
《中国工业医学杂志》
CAS
2017年第1期18-20,共3页
Chinese Journal of Industrial Medicine
