摘要
目的探讨血清趋化因子及氧化与抗氧化生物标志物在职业性矽肺(简称“矽肺”)中的作用。方法采用方便抽样方法,选择58例矽肺壹期患者为研究对象。采用酶联免疫吸附法检测其血清中核因子E2相关因子(Nrf2)、血红素氧合酶-1(HO-1)和8-异前列腺素F2α(8-iso-PGF2α)水平,采用化学比色法检测血清中脂质过氧化物(LPO)和总抗氧化能力(T-AOC),采用Luminex流式荧光技术检测血清中γ-干扰素诱导蛋白10(IP-10)、巨噬细胞炎症蛋白(MIP)-1α、MIP-1β和巨噬细胞衍生趋化因子(MDC)水平;对上述指标进行因子分析。结果9项生物标志物原始指标信息量提取率为58.5%~96.5%。提取到4个公因子分别为Nrf2抗氧化通路、辅助性T细胞(Th)1优势趋化、总的氧化/抗氧化平衡和Th2优势趋化,其方差贡献率分别为32.2%、19.1%、16.4%和11.8%,累计方差贡献率为79.5%。结论氧化/抗氧化失衡和Th选择性优势趋化均参与矽肺的发生发展;其中,Nrf2抗氧化通路的作用最为关键。
Objective To investigate the role of serum chemokines,and oxidative and antioxidant biomarkers in occupational silicosis(hereinafter referred to as silicosis).Methods A total of 58 patients with stageⅠsilicosis were selected as the research subjects using convenient sampling method.The serum levels of nuclear factor erythroid-2 related factor 2(Nrf2),heme oxygenase-1(HO-1)and 8-isoprstaglandin F2α(8-iso-PGF2α)were determined by enzyme-linked immunesorbent assay.The serum levels of lipid peroxide(LPO)and total antioxidant capacity(T-AOC)were determined by chemistry colorimetric method.Luminex flow fluorescence technology was used to detect the serum levels of interferon-γinducible protein-10(IP-10),macrophage inflammatory protein(MIP)-1α,MIP-1βand macrophage-derived chemokine(MDC).The above indicators were analyzed by factor analysis.Results The information extraction rate of the original indicators of the nine biomarkers was 58.5%-96.5%.Four common factors were extracted,including Nrf2 antioxidant signaling pathway,helper T cell(Th)1 dominant chemotaxis,the total oxidation/antioxidant balance,and Th2 dominant chemotaxis,whose variance contribution rates were 32.2%,19.1%,16.4%,and 11.8%,respectively,and the cumulative variance contribution rate was 79.5%.Conclusion Both the oxidant-antioxidant disturbance and the dominance chemotaxis are involved in the occurrence and development of silicosis,and the Nrf2 antioxidant signaling pathway plays the most critical role.
作者
邹吉敏
袁宝军
王冬梅
李超
高利常
佟艳艳
崔晶晶
ZOU Ji-min;YUAN Bao-jun;WANG Dong-mei;LI Chao;GAO Li-chang;TONG Yan-yan;CUI Jing-jing(Department of Clinical Laboratory,North China University of Science and Technology Hospital,Tangshan,Hebei 063210,China;不详)
出处
《中国职业医学》
CAS
北大核心
2022年第6期687-691,共5页
China Occupational Medicine
