摘要
目的研究加味开心散对多因素神经损伤痴呆小鼠学习记忆能力的影响及作用机制。方法利用多因素神经损伤构建痴呆小鼠模型,给以加味开心散水提物与水醇共提物,以石杉碱甲(0.05mg/kg)作为阳性对照药物,连续灌胃10d;采用Morris水迷宫与主动避暗实验等行为学实验,评价加味开心散对多因素神经损伤痴呆小鼠学习记忆能力的影响。同时测定受试小鼠大脑皮层中胆碱酯酶(ChE)、超氧化物歧化酶(SOD)活力,神经生长因子(NGF)及其前体蛋白表达水平,探索其作用机制。结果行为学测试结果显示加味开心散可明显缩短小鼠Morris水迷宫定位导航潜伏期,增加空间探索中穿越平台次数,减少上台前游泳距离;降低主动回避实验中明暗箱穿梭错误次数。降低ChE活性,提高NGF的表达水平。水醇共提物效果优于水提物。结论加味开心散可明显增强多因素神经损伤痴呆小鼠的学习记忆能力,其作用的机制可能与抑制乙酰胆碱降解,提升神经因子表达有关。
OBJECTIVE To evaluate the effect of Jiawei Kaixin San（JWKXS） on learning and memory abilities of multiple neuronal damage induced dementia mice and explore its mechanism. METHODS Dementia mice model was established via multiple neuronal damages, then the model mice were treated with water extracts, and water and ethanol combined extracts of JWKXS for 10 d, while huperzine （0.05 mg/kg） was applied as positive drug. The abilities of learning and memory were evalu- ated by behavioral tests, such as Morris water maze test and active avoidance test. In addition, cholinesterase（ChE） activities, superoxide dismutase（SOD） and protein expression levels of nerve growth factor（NGF） with its precursor in cerebral cortex of model mice were detected. RESULTS Behavioral tests showed JWKXS obviously shortened the incubation time of navigation and positioning, increased the times across platform and decreased swimming distances in Morris water maze test. In active a- voidance test, the shuttle error numbers of model mice decreased with the treatment of JWKXS. In cerebral cortex, ChE activ- ities were down-regulated while NGF levels were up-regulated. The effects of water and ethanol combined extract were better than the water extracts. CONCLUSION JWKXS can significantly improve the learning and memory ability of dementia mice induced by multiple neuronal damage. Its mechanism might he related to the inhibition of acetyleholine degradation and up-reg- ulation of nerve factor expression.
出处
《南京中医药大学学报》
CAS
CSCD
北大核心
2017年第1期44-48,共5页
Journal of Nanjing University of Traditional Chinese Medicine
基金
江苏省高校自然科学重大研究项目(15KJA360002)
国家自然科学基金(81303190)
江苏省自然科学基金(BK20141462)
江苏高校品牌专业建设工程资助项目(PPZY2015A070)
江苏高校优势学科建设工程资助项目(YSXK-2014)
江苏省大学生创新训练计划项目(201510315086x)
江苏省中药资源产业化过程协同创新中心资助项目(FJGJS-2015-10)
关键词
加味开心散
多因素神经损伤痴呆小鼠模型
学习记忆
Jiawei Kaixin San
the multi-factor damage dementia mice model
learning and memory
