摘要
目的:研究石菖蒲水提取液及其挥发油对淀粉样β蛋白25-35(Aβ25-35)二级结构的影响及机制。方法:运用圆二色谱表征Aβ25-35二级结构的变化。结果:Aβ25-35孵育不同时间及用石菖蒲水提取液及其挥发油分别干预后,它的CD图形发生了明显改变。Aβ25-35水溶液孵育30 m in全部为α-螺旋;孵育24h,α-螺旋向着其它的二级结构转化,其中α-螺旋10.10%,β-折叠18.40%;孵育7 d,主要以β-折叠和不规则卷曲为主,含量分别是27.00%和56.40%。用石菖蒲水提取液及其挥发油分别与Aβ25-35水溶液共同孵育后,α-螺旋结构明显增多,其中以100%水提取液孵育7 d和100%挥发油孵育7 d的效果最好,α-螺旋含量达到100%。结论:一定浓度石菖蒲水提取液及其挥发油一定时间内能够有效地阻止了Aβ25-35由α-螺旋向β-折叠转化。
AIM： To study the effect and the mechanism of rhizoma acori graminei＇s water extract and its naphtha on the secondary structure of amyloid beta - protein 25 - 35 （ abeta 25 - 35 ）. METHODS ： Abeta 25 - 35 protein was cultured in PBS for 30 min, 24 h, 7 d in 37℃, then the change of secondary structure in abeta 25 - 35 was measured by circular dichroism （CD）. Under the same condition, abeta 25 -35 protein was cultured with different concentrations of RAG＇s water extract and its naphtha instead of PBS, its secondary structure was also observed with CD. RESULTS： Obvious changes were observed between the CD graphs： when cultured in PBS for 30 min, the secondary structure of abeta 25 - 35 protein all were α- helix; but after 24 h, its secondary structure change, only 10. 10% α-helix and 18.40% β - sheet was observed. When cultured for 7 days, its secondary structure mainly were β - sheet （ 27.00% ） and random （56.40%）. After cultured with RAG＇s water extract and its naphtha, α- helix structure increased evidently, and the result showed that the best effect was when it is cultured with 100% RAG＇s water extract and 100% naphtha for 7 days, its secondary structure all converted to α- helix. CONCLUSION： Definite concentration of RAG＇s water extract and its naphtha effectively prevented abeta 25 - 35 protein＇s secondary structure change from α- helix to β - sheet in definite times.
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2007年第2期352-355,共4页
Chinese Journal of Pathophysiology
基金
暨南大学引进优秀人才科研启动基金资助项目(No.51292033)
关键词
阿尔茨海默病
淀粉样Β蛋白
石菖蒲
圆二色性
Alzheimer disease
Amyloid beta- protein
Rhizoma acori graminei
Circular dichroism
