摘要
以多糖为软模板,可以制备高效低毒的纳米硒-多糖复合物。本文以废弃的蛹虫草培养基质中提取的水溶性多糖为原料,研究了蛹虫草基质多糖纳米硒复合物的制备工艺及抗肿瘤活性。实验结果表明,在多糖浓度为10 mg/m L、亚硒酸浓度为0.4 mol/L、混合时间为4 h、滴加速度为1.5 r/min的条件下,可以制备出形貌理想、粒径适宜、稳定性良好的蛹虫草基质多糖纳米硒复合物。该复合物对Caco-2肿瘤细胞的生长具有一定抑制效果,且对细胞毒作用呈现出剂量依赖效应。
Polysaecharides was used as soft template to prepare polysaccharide/Nano-Selenium complexes, which had high biological activity and low toxicity.The preparation procedure of Nano-Se/Cordyceps militaris stroma polysaccharides complexes was investigated by single-factor experiment and orthogonal experimental design, and the water-soluble polysaccharide which was exacted from the abandoned cordyceps militaris culture medium was used as the raw material.The results showed that the optimal reaction conditionswere as follows：the polysaccharide concentration was 10 mg/mL, selenite concentration was 0.4 mot/L, the mixing time was 4 hours, the preparation conditions of dropping was 1.5 r/min. Under the optimal condiction, the ideal morphology,ideal particle size, stable and good nano selenium/Cordyceps militaris stroma polysaceharide complex particles （CMSP-SeNPs） could be successfully prepared.The antitumor experiment results showed that CMSP-SeNPs could inhibit the proliferation of Caeo-2 cells,which presented a certain dose-response relationship.
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第5期49-53,59,共6页
Science and Technology of Food Industry
基金
国家自然科学基金项目(21376112
31471699)
国家高技术研究发展计划项目(2012AA021701)
江苏省自然科学基金(BK20131344
BK20141081)
2015年江苏省大学生实践创新训练计划项目(201511460007Z)
南京晓庄学院校科研项目(2012NXY08)
关键词
蛹虫草
多糖
硒
纳米
抗肿瘤
Cordyceps militaris
polysaccharide
Selenium
Nano
antitumor
