摘要
目的研究microRNA-106b（miR-106b）在TC组织中的表达及其临床意义，并探讨其对TC细胞增殖及凋亡的影响。方法采用qRT-PCR检测51例TC及相应癌旁组织中miR-106b的表达并分析其与TC患者临床病理特征的相关性，并利用miR-106b过表达质粒及抑制质粒改变其在TC细胞中的表达，利用BrdU细胞增殖分析及流式细胞仪检测细胞增殖及凋亡的变化。结果miR-106b在TC组织中的表达水平显著低于相应癌旁组织（0.36±0.029 vs 0.98±0.034，P=0.004）。miR-106b表达与肿瘤大小（P=0.002）及肿瘤数目（P=0.041）有关。miR-106b抑制剂能显著降低miR-106b在TPC-1细胞的表达水平（0.96±0.025 vs 0.29±0.032，P=0.001），并能显著增强细胞增殖能力（89.33±5.67 vs 136.67±10.33，P=0.03），减少细胞凋亡（16.33±3.20 vs 7.67±2.45，P=0.04）；miR-106b类似物能显著增加miR-106b在TPC-1细胞的表达水平（0.99±0.02 vs 3.19±0.68，P=0.002），并能显著减弱细胞增殖能力（98.00±4.65 vs 76.33±2.87, P=0.03），增加细胞凋亡（22.54±2.13 vs 32.45±4.34，P=0.04）。结论miR-106b在TC组织中的表达水平显著低于相应癌旁组织，其表达降低与TC患者的不良临床病理特征相关；miR-106b能减弱细胞增殖能力，增加细胞凋亡。提示miR-106b在TC的发生发展中发挥重要作用。
ObjectiveTo investigate clinical significance and function of microRNA-106b （miR-106b） in retinoblastoma tissues and cells.MethodsWe detected miR-106b expression in 51 samples of thyroid cancer and the adjacent non-tumor tissues using qRT-PCR. The expression of miR-106b was altered by corresponding vectors in thyroid cancer cells, and then BrdU cell proliferation and flow cytometry assay were performed to examine the proliferation and apoptosis of thyroid cancer cells.Results The expression of miR-106b in thyroid cancer tissues was significantly lower than that in normal tumor-adjacent tissues （0.36±0.029 vs 0.98±0.034, P=0.004） . MiR-106b expression in tumor tissues was significantly associated with tumor size and tumor number. MiR-106b was obviously inhibited by miR-106b inhibitor in PTC-1 cells （0.96±0.025 vs 0.29±0.032, P=0.001） , and inhibition of miR-106b resulted in significantly increased proliferation （89.33±5.67 vs 136.67±10.33, P=0.03） and decreased apoptosis （16.33±3.20 vs 7.67±2.45, P=0.04） . On the contrast, over-expression of miR-106b using miR-106b mimics led to significantly decreased proliferation （98.00±4.65 vs 76.33±2.87, P=0.03） and increased apoptosis （22.54±2.13 vs 32.45±4.34, P=0.04） .ConclusionsDecreased expression of miR-106b is correlated with the adverse clinicopathological features of thyroid cancer. MiR-106b can inhibit cell proliferation and apoptosis of thyroid cancer cells, suggesting miR-106b plays a suppressive role in development and progression of thyroid cancer.
出处
《中华内分泌外科杂志》
CAS
2017年第1期60-64,共5页
Chinese Journal of Endocrine Surgery
