摘要
目的研究东北汉族群体的19个Y-STR基因座的遗传多态性和东北亚15个群体间的遗传关系,探讨其法医学和群体遗传学研究的应用价值。方法采用Y-filer复合扩增体系对200例东北汉族无血缘关系的男性个体的19个Y-STR基因座进行扩增,应用ABI310分析仪进行基因分型,采用Arelquin3.11、Mega4.1和SPSS17.0软件进行群体间的AMOVA分析、聚类分析和多维标度分析。结果东北汉族群体的19个Y-STR基因座的基因多态性普遍较高,共检出200个单倍型,单倍型多态性在0.350 9(DYS391)~0.971 1(DYS385a/b),适合应用于法医学个体识别和亲权鉴定;东北亚15个群体间存在差异,遗传距离在0.000 9~0.643 2,群体间聚类分析和多维度分析结果与民族起源和迁徙史基本相一致。结论东北汉族群体的19个Y-STR基因座的基因多态性普遍较高,适合应用于法医学个体识别和亲权鉴定中。
Objective To investigate the polymorphisms of the 19Y-STR loci in the Han population in Northeast China,and analyze the genetic relationships of 15 ethnic groups in northeast Asia region,and to evaluate their forensic value and population genetic value.Methods The 19Y-STR unrelated Han males in 200 unrelated Han males in Northeast China were amplified with the Y-filer system,and the PCR products were analyzed by the 310 Genetic Analyzer.The AMOVA analysis,cluster analysis and MDS analysis were calculated by Arelquin3.11,Mega4.1and SPSS17.0.Results The polymorphisms of 19Y-STR loci in the Han population in Northeast China had generally higher gene diversity value which were ranged from 0.350 9（DYS391）to 0.971 1（DYS385a/b）,and totally 200 haplotypes were observed.The 19Y-STR loci displayed high genetic polymorphisms in the Han population in Northeast China,indicating that these 19 loci were useful genetic markers for forensic personal identification and paternity testing.There were distinctions among 15 ethnic groups.The genetic distance between 15 ethnic groups were ranged from 0.000 9to 0.643 2,and the conclusion of cluster analysis and MDS analysis were similar to the ethnogeny research and ethnic migration history.Conclusion The 19Y-STR loci displayed high genetic polymorphisms in the Han population in Northeast China,and these 19 loci were useful genetic markers for forensic personal identification and paternity testing.
出处
《重庆医学》
CAS
北大核心
2017年第5期658-660,664,共4页
Chongqing medicine
关键词
Y染色体
短串联重复序列
多态性
汉族
东北亚
Y-Chromosome
short tandem repeats
polymorphism
Han population
northeast Asia
