摘要
Y-STR技术现已成为法庭科学领域重要的检验鉴定方法。目前单拷贝Y-STR基因座广受瞩目,然而,多拷贝Y-STR基因座在Y染色体上有2~4个拷贝,多态性更高,可望作为Y-STR鉴定的重要补充。迄今为止,已报道的多拷贝基因座达几十种,其中包含多个快速突变的基因座。利用各地的人群样本,国内外陆续开展了针对少数多拷贝基因座的遗传多态性研究,其中,国内的此类研究多集中于河南汉族人群,全面的研究工作仍待推进。现有的Y-STR复合扩增体系多数只包含2~3个多拷贝基因座,将大量多拷贝Y-STR基因座纳入复合扩增体系将可能提升系统的检验效能。本文从多拷贝Y-STR基因座的结构特点、突变特点、遗传多态性及复合扩增研究等多方面进行了综合评述,并提出了未来多拷贝Y-STR基因座的研究方向,为Y-STR技术更好地应用于法庭科学领域进行了初步探索。
As one of the most important genetic markers used in forensic science,Y-STR has been widely applied,with its single-copied loci being paid extraordinary attention.However,multi-copied Y-STR loci generally contain 2-4 copies and display high genetic polymorphism,therefore allowing them to be an important replenishment to the current technique.Although there are reports on dozens of multi-copied Y-STR loci,including multiple rapidly-mutating loci,yet these researches only focus a few of them on the genetic diversity.The related studies in China have been mostly carried out on Han population,mainly from Henan province,therefore expecting them to be extended to a wider scope.The present-used multiplexes usually include only 2-3 multi-copied loci.Thus,the inclusion of more multi-copied loci might improve their discrimination power.In this paper,a comprehensive review was made on the studies about the multi-copied Y-STR locus’structure,mutation,allelic frequency and the involved multiplex construction,finally pointing out the directions for future research.
作者
尚蕾
莫晓婷
杨帆
张建
余政梁
马新
赵兴春
李万水
SHANG Lei;MO Xiaoting;YANG Fan;ZHANG Jian;YU Zhengliang;MA Xin;ZHAO Xingchun;LI Wanshui(Institute of Forensic Science,Ministry of Public Security,Beijing100038,China)
出处
《刑事技术》
2018年第2期97-103,共7页
Forensic Science and Technology
基金
"十三五"国家重点研发计划项目(No.2017YFC0803507)
公安部技术研究计划(No.2016JSYJC11
2016JSYJC14)
中央级公益性科研院所基本科研业务费专项资金项目(No.2016JB040)
