摘要
用胰蛋白酶对脱脂鲤鱼卵进行酶解能够得到具有钙结合活性的酶解物。最佳水解条件为:温度49℃,加酶量3 000(U/g底物),底物浓度2%,p H 9.0。用氢氧化钠对鱼卵进行适度的脱磷处理,当脱磷率为30.39%时,能显著提高鱼卵的水解度及酶解液钙结合能力,胰蛋白酶酶解12 h后,水解度为31.05%,酶解液钙结合能力为0.67(mmol Ca/g蛋白)。
The enzymolysis to defatted carps with trypsin protease could obtain enzymatic hydrolysate with calcium binding activity. The best hydrolysis condition was as follows：49 ℃,enzyme dosage 3 000（U/g substrate）,substrate concentration 2 %,and p H 9.0. Moderate dephosphorization treatment on carp eggs（rate：30.39 %）could significantly improve the degree of hydrolysis and the Ca-binding activity of enzymatic hydrolysate. After 12 h of hydrolysis by trypsin,the degree of hydrolysis was up to 31.05 % and the Ca-binding activity was 0.67（mmol Ca/g protein）.
作者
黄海
李八方
曾名湧
HUANG Hai LI Ba-fang ZENG Ming-yong(College of Food Engineering, Qinzhou University, Qinzhou 535011, Guangxi, China Guangxi Colleges and Universities Key Laboratory of Development and High-value Utilization of Beibu Gulf Seafood Resource, Qinzhou 535011, Guangxi, China College of Food Science and Engineering, Ocean University of China, Qingdao 266003, Shandong, China)
出处
《食品研究与开发》
CAS
北大核心
2017年第1期73-77,共5页
Food Research and Development
基金
国家星火计划项目(2012GA740048)
关键词
鲤鱼卵
酶解制备
钙结合活性
carp eggs
enzymatic preparation
Ca-binding activity
